Academic Journal
Suppression of Multiclade R5 and X4 Human Immunodeficiency Virus Type-1 Infections by a Coreceptor-Based Anti-HIV Strategy
| العنوان: | Suppression of Multiclade R5 and X4 Human Immunodeficiency Virus Type-1 Infections by a Coreceptor-Based Anti-HIV Strategy |
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| المؤلفون: | Nakayama, Daisuke, Misumi, Shogo, Mukai, Ryouzaburo, Tachibana, Kuniomi, Umeda, Mamoru, Shibata, Hideaki, Takamune, Nobutoki, Shoji, Shozo |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2005 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Regular Papers |
| الوصف: | A cyclic chimeric dodecapeptide (cCD) mimicking the conformation-specific domains of CCR5 and CXCR4 was prepared in which Gly-Asp links the amino and carboxyl termini of two combined pentapeptides (S169–G173 of CCR5; E179-R183 of CXCR4) derived from human immunodeficiency virus type-1 (HIV-1) coreceptors. The immunization of Balb/c mice with cCD conjugated with a multiple-antigen peptide (cCD-MAP) induced seven cCD-specific monoclonal antibodies (mAbs, CPMAb-I to -VII) that reacted with native CCR5 and CXCR4. Among the tested mAbs, CPMAb-I and -II potently inhibited the infection of both the R5 and X4 laboratory strains. CPMAb-III and -VI were effective against only R5 laboratory strains, and also against some X4 and R5 primary isolates. CPMAb-IV and -V had potent antiviral activities against the R5 and X4 primary isolates. In particular, CPMAb-VII was protective against not only R5 and X4 laboratory strains, but also most of the R5 and X4 primary isolates. Moreover, cCD-MAP immunization also induced antibodies that were effective against R5 and X4 multiclade HIV-1 isolates in vitro in two of three cynomolgus monkeys. Taken together, the results suggest that cCD-MAP is a candidate multiclade immunogen that can be used to block multiclade R5 and X4 HIV-1 infections. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://jb.oxfordjournals.org/cgi/content/short/138/5/571; http://dx.doi.org/10.1093/jb/mvi165 |
| DOI: | 10.1093/jb/mvi165 |
| الاتاحة: | http://jb.oxfordjournals.org/cgi/content/short/138/5/571 https://doi.org/10.1093/jb/mvi165 |
| Rights: | Copyright (C) 2005, Japanese Biochemical Society |
| رقم الانضمام: | edsbas.FC4AD470 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/jb/mvi165 |
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