Academic Journal
A genome scale overexpression screen to reveal drug activity in human cells
| العنوان: | A genome scale overexpression screen to reveal drug activity in human cells |
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| المؤلفون: | Arnoldo, Anthony, Kittanakom, Saranya, Heisler, Lawrence E, Mak, Anthony B, Shukalyuk, Andrey I, Torti, Dax, Moffat, Jason, Giaever, Guri, Nislow, Corey |
| بيانات النشر: | BioMed Central |
| سنة النشر: | 2014 |
| المجموعة: | University of British Columbia: cIRcle - UBC's Information Repository |
| الوصف: | Target identification is a critical step in the lengthy and expensive process of drug development. Here, we describe a genome-wide screening platform that uses systematic overexpression of pooled human ORFs to understand drug mode-of-action and resistance mechanisms. We first calibrated our screen with the well-characterized drug methotrexate. We then identified new genes involved in the bioactivity of diverse drugs including antineoplastic agents and biologically active molecules. Finally, we focused on the transcription factor RHOXF2 whose overexpression conferred resistance to DNA damaging agents. This approach represents an orthogonal method for functional screening and, to our knowledge, has never been reported before. ; Pharmaceutical Sciences, Faculty of ; Non UBC ; Reviewed ; Faculty |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | Genome Medicine. 2014 Apr 29;6(4):32; http://hdl.handle.net/2429/56670 |
| DOI: | 10.1186/gm549 |
| الاتاحة: | http://hdl.handle.net/2429/56670 https://doi.org/10.1186/gm549 |
| Rights: | Attribution 4.0 International (CC BY 4.0) ; http://creativecommons.org/licenses/by/4.0/ ; Arnoldo et al.; licensee BioMed Central Ltd. |
| رقم الانضمام: | edsbas.FBD06A4B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/gm549 |
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