Academic Journal
Identification of d-arabinan-degrading enzymes in mycobacteria
| العنوان: | Identification of d-arabinan-degrading enzymes in mycobacteria |
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| المؤلفون: | Al-Jourani, Omar, Benedict, Samuel T., Ross, Jennifer, Layton, Abigail J., van der Peet, Phillip, Marando, Victoria M., Bailey, Nicholas P., Heunis, Tiaan, Manion, Joseph, Mensitieri, Francesca, Franklin, Aaron, Abellon-Ruiz, Javier, Oram, Sophia L., Parsons, Lauren, Cartmell, Alan, Wright, Gareth S.A., Baslé, Arnaud, Trost, Matthias, Henrissat, Bernard, Munoz-Munoz, Jose, Hirt, Robert P., Kiessling, Laura L., Lovering, Andrew L., Williams, Spencer J., Lowe, Elisabeth C., Moynihan, Patrick J. |
| المصدر: | Al-Jourani , O , Benedict , S T , Ross , J , Layton , A J , van der Peet , P , Marando , V M , Bailey , N P , Heunis , T , Manion , J , Mensitieri , F , Franklin , A , Abellon-Ruiz , J , Oram , S L , Parsons , L , Cartmell , A , Wright , G S A , Baslé , A , Trost , M , Henrissat , B , Munoz-Munoz , J , Hirt , R P , Kiessling , L L , .... |
| سنة النشر: | 2023 |
| المجموعة: | Technical University of Denmark: DTU Orbit / Danmarks Tekniske Universitet |
| مصطلحات موضوعية: | /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being, name=SDG 3 - Good Health and Well-being |
| الوصف: | Bacterial cell growth and division require the coordinated action of enzymes that synthesize and degrade cell wall polymers. Here, we identify enzymes that cleave the d-arabinan core of arabinogalactan, an unusual component of the cell wall of Mycobacterium tuberculosis and other mycobacteria. We screened 14 human gut-derived Bacteroidetes for arabinogalactan-degrading activities and identified four families of glycoside hydrolases with activity against the d-arabinan or d-galactan components of arabinogalactan. Using one of these isolates with exo-d-galactofuranosidase activity, we generated enriched d-arabinan and used it to identify a strain of Dysgonomonas gadei as a d-arabinan degrader. This enabled the discovery of endo- and exo-acting enzymes that cleave d-arabinan, including members of the DUF2961 family (GH172) and a family of glycoside hydrolases (DUF4185/GH183) that display endo-d-arabinofuranase activity and are conserved in mycobacteria and other microbes. Mycobacterial genomes encode two conserved endo-d-arabinanases with different preferences for the d-arabinan-containing cell wall components arabinogalactan and lipoarabinomannan, suggesting they are important for cell wall modification and/or degradation. The discovery of these enzymes will support future studies into the structure and function of the mycobacterial cell wall. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://orbit.dtu.dk/en/publications/0c3775de-8782-4582-ad16-130a8aaaf38f |
| DOI: | 10.1038/s41467-023-37839-5 |
| الاتاحة: | https://orbit.dtu.dk/en/publications/0c3775de-8782-4582-ad16-130a8aaaf38f https://doi.org/10.1038/s41467-023-37839-5 https://backend.orbit.dtu.dk/ws/files/319634377/s41467_023_37839_5.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.FB58FD3F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-023-37839-5 |
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