Academic Journal
Solid‐phase peptide synthesis in 384‐well plates
| العنوان: | Solid‐phase peptide synthesis in 384‐well plates |
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| المؤلفون: | Schüttel, Mischa, Will, Edward, Sangouard, Gontran, Zarda, Anne, Habeshian, Sevan, Nielsen, Alexander L., Heinis, Christian |
| المساهمون: | Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, European Research Council |
| المصدر: | Journal of Peptide Science ; volume 30, issue 4 ; ISSN 1075-2617 1099-1387 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2024 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Newer solid‐phase peptide synthesis and release strategies enable the production of short peptides with high purity, allowing direct screening for desired bioactivity without prior chromatographic purification. However, the maximum number of peptides that can currently be synthesized per microplate reactor is 96, allowing the parallel synthesis of 384 peptides in modern devices that have space for 4 microplate reactors. To synthesize larger numbers of peptides, we modified a commercially available peptide synthesizer to enable the production of peptides in 384‐well plates, which allows the synthesis of 1,536 peptides in one run (4 × 384 peptides). We report new hardware components and customized software that allowed for the synthesis of 1,536 short peptides in good quantity (average > 0.5 μmol), at high concentration (average > 10 mM), and decent purity without purification (average > 80%). The high‐throughput peptide synthesis, which we developed with peptide drug development in mind, may be widely used for peptide library synthesis and screening, antibody epitope scanning, epitope mimetic development, or protease/kinase substrate screening. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/psc.3555 |
| الاتاحة: | http://dx.doi.org/10.1002/psc.3555 https://onlinelibrary.wiley.com/doi/pdf/10.1002/psc.3555 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.FB56ED2D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/psc.3555 |
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