Academic Journal
Assessment of glutathione/glutathione disulphide ratio and S-glutathionylated proteins in human blood, solid tissues, and cultured cells
| العنوان: | Assessment of glutathione/glutathione disulphide ratio and S-glutathionylated proteins in human blood, solid tissues, and cultured cells |
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| المؤلفون: | GIUSTARINI, DANIELA, ALOISI, ANNA MARIA, SANTUCCI, ANNALISA, ROSSI, RANIERI, Colombo, Graziano, Garavaglia, Maria Lisa, Astori, Emanuela, Portinaro, Nicola Marcello, Reggiani, Francesco, Badalamenti, Salvatore, Milzani, Aldo, Dalle donne, Isabella |
| المساهمون: | Giustarini, Daniela, Colombo, Graziano, Garavaglia, Maria Lisa, Astori, Emanuela, Portinaro, Nicola Marcello, Reggiani, Francesco, Badalamenti, Salvatore, Aloisi, ANNA MARIA, Santucci, Annalisa, Rossi, Ranieri, Milzani, Aldo, Dalle donne, Isabella |
| سنة النشر: | 2017 |
| المجموعة: | Università degli Studi di Siena: USiena air |
| مصطلحات موضوعية: | Anti-GSH antibodie, Biotinylation, Clickable glutathione, Fluorescent probe, Glutathione, Proteomic, S-glutathionylated protein, Thiol alkylation, Biochemistry, Physiology (medical) |
| الوصف: | Glutathione (GSH) is the major non-protein thiol in humans and other mammals, which is present in millimolar concentrations within cells, but at much lower concentrations in the blood plasma. GSH and GSH-related enzymes act both to prevent oxidative damage and to detoxify electrophiles. Under oxidative stress, two GSH molecules become linked by a disulphide bridge to form glutathione disulphide (GSSG). Therefore, assessment of the GSH/GSSG ratio may provide an estimation of cellular redox metabolism. Current evidence resulting from studies in human blood, solid tissues, and cultured cells suggests that GSH also plays a prominent role in protein redox regulation via S -glutathionylation, i.e., the conjugation of GSH to reactive protein cysteine residues. A number of methodologies that enable quantitative analysis of GSH/GSSG ratio and S-glutathionylated proteins (PSSG), as well as identification and visualization of PSSG in tissue sections or cultured cells are currently available. Here, we have considered the main methodologies applied for GSH, GSSG and PSSG detection in biological samples. This review paper provides an up-to-date critical overview of the application of the most relevant analytical, morphological, and proteomics approaches to detect and analyse GSH, GSSG and PSSG in mammalian samples as well as discusses their current limitations. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/28807817; info:eu-repo/semantics/altIdentifier/wos/WOS:000411829300032; volume:112; firstpage:360; lastpage:375; numberofpages:16; journal:FREE RADICAL BIOLOGY & MEDICINE; http://hdl.handle.net/11365/1019269; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85027510714; www.elsevier.com/locate/freeradbiomed |
| DOI: | 10.1016/j.freeradbiomed.2017.08.008 |
| الاتاحة: | http://hdl.handle.net/11365/1019269 https://doi.org/10.1016/j.freeradbiomed.2017.08.008 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.FA725C6F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.freeradbiomed.2017.08.008 |
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