التفاصيل البيبلوغرافية
| العنوان: |
Data_Sheet_1_Identification of Functional Interactome of Colistin Resistance Protein MCR-1 in Escherichia coli.docx |
| المؤلفون: |
Hui Li (32376), Yingyu Wang (732687), Qiyan Chen (9238376), Xi Xia (401036), Jianzhong Shen (261666), Yang Wang (5921), Bing Shao (359353) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Microbiology, Microbial Genetics, Microbial Ecology, Mycology, MCR-1, colistin resistance, interactome, co-immunoprecipitation, E. coli |
| الوصف: |
The emergence and worldwide dissemination of plasmid-mediated colistin resistance gene mcr-1 has attracted global attention. The MCR-1 enzyme mediated colistin resistance by catalyzing phosphoethanolamine (PEA) transfer onto bacterial lipid A. However, the interaction partners of MCR-1 located in membrane protein in E. coli are unknown. Co-immunoprecipitation (Co-IP) and Mass Spectrometry were performed to define the interacting proteins of MCR-1. A total of three different anti-MCR-1 monoclonal antibody (mAbs) were prepared and 3G4 mAb was selected as the bait protein by compared their suitability for Co-IP. We identified 53, 13, and 14 interacting proteins in E. coli BL21 (DE3) (pET28a-mcr-1), E. coli BL21 (DE3) (pET28a-mcr-1-200), and E. coli DH5α (pUC19-mcr-1), respectively. Six proteins, including the stress response proteins DnaK (chaperone protein) and SspB (stringent starvation protein B), the transcriptional regulation protein H-NS, and ribosomal proteins (RpsE, RpsJ, and RpsP) were identified in all these three strains. These MCR-1-interacting proteins were mainly involved in ribosome and RNA degradation, suggesting that MCR-1 influences the protein biosynthesis through the interaction with ribosomal protein. Multidrug efflux pump AcrA and TolC were important interacting membrane proteins of MCR-1 referred to drug efflux during the PEA modification of the bacterial cell membrane. Overall, we firstly identified the functional interactome profile of MCR-1 in E. coli and discovered that two-component AcrA-TolC multidrug efflux pump was involved in mcr-1-mediated colistin resistance. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/Data_Sheet_1_Identification_of_Functional_Interactome_of_Colistin_Resistance_Protein_MCR-1_in_Escherichia_coli_docx/13635632 |
| DOI: |
10.3389/fmicb.2020.583185.s001 |
| الاتاحة: |
https://doi.org/10.3389/fmicb.2020.583185.s001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.FA71BF79 |
| قاعدة البيانات: |
BASE |