Academic Journal
Species-specific PCR assay for the detection of Babesia odocoilei
| العنوان: | Species-specific PCR assay for the detection of Babesia odocoilei |
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| المؤلفون: | Burgess, Hilary J., Lockerbie, Betty P., Ayalew, Lisanework E., Dibernardo, Antonia, Hrazdilová, Kristýna, Modry, David, Bollinger, Trent K. |
| المساهمون: | Ministry of Education, Youth and Sports of the Czech Republic |
| المصدر: | Journal of Veterinary Diagnostic Investigation ; volume 33, issue 6, page 1188-1192 ; ISSN 1040-6387 1943-4936 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2021 |
| الوصف: | We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/10406387211032927 |
| الاتاحة: | https://doi.org/10.1177/10406387211032927 https://journals.sagepub.com/doi/pdf/10.1177/10406387211032927 https://journals.sagepub.com/doi/full-xml/10.1177/10406387211032927 |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.F9CD7B11 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/10406387211032927 |
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