Academic Journal
Gene Expression Profiling of Bronchoalveolar Lavage Cells Preceding a Clinical Diagnosis of Chronic Lung Allograft Dysfunction.
| العنوان: | Gene Expression Profiling of Bronchoalveolar Lavage Cells Preceding a Clinical Diagnosis of Chronic Lung Allograft Dysfunction. |
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| المؤلفون: | S Samuel Weigt, Xiaoyan Wang, Vyacheslav Palchevskiy, Aric L Gregson, Naman Patel, Ariss DerHovanessian, Michael Y Shino, David M Sayah, Shirin Birjandi, Joseph P Lynch, Rajan Saggar, Abbas Ardehali, David J Ross, Scott M Palmer, David Elashoff, John A Belperio |
| المصدر: | PLoS ONE, Vol 12, Iss 1, p e0169894 (2017) |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2017 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | BACKGROUND:Chronic Lung Allograft Dysfunction (CLAD) is the main limitation to long-term survival after lung transplantation. Although CLAD is usually not responsive to treatment, earlier identification may improve treatment prospects. METHODS:In a nested case control study, 1-year post transplant surveillance bronchoalveolar lavage (BAL) fluid samples were obtained from incipient CLAD (n = 9) and CLAD free (n = 8) lung transplant recipients. Incipient CLAD cases were diagnosed with CLAD within 2 years, while controls were free from CLAD for at least 4 years following bronchoscopy. Transcription profiles in the BAL cell pellets were assayed with the HG-U133 Plus 2.0 microarray (Affymetrix). Differential gene expression analysis, based on an absolute fold change (incipient CLAD vs no CLAD) >2.0 and an unadjusted p-value ≤0.05, generated a candidate list containing 55 differentially expressed probe sets (51 up-regulated, 4 down-regulated). RESULTS:The cell pellets in incipient CLAD cases were skewed toward immune response pathways, dominated by genes related to recruitment, retention, activation and proliferation of cytotoxic lymphocytes (CD8+ T-cells and natural killer cells). Both hierarchical clustering and a supervised machine learning tool were able to correctly categorize most samples (82.3% and 94.1% respectively) into incipient CLAD and CLAD-free categories. CONCLUSIONS:These findings suggest that a pathobiology, similar to AR, precedes a clinical diagnosis of CLAD. A larger prospective investigation of the BAL cell pellet transcriptome as a biomarker for CLAD risk stratification is warranted. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC5245825?pdf=render; https://doaj.org/toc/1932-6203; https://doaj.org/article/51c2cfff00ed41e99d5e67da95b3a38c |
| DOI: | 10.1371/journal.pone.0169894 |
| الاتاحة: | https://doi.org/10.1371/journal.pone.0169894 https://doaj.org/article/51c2cfff00ed41e99d5e67da95b3a38c |
| رقم الانضمام: | edsbas.F8F69FE0 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0169894 |