التفاصيل البيبلوغرافية
| العنوان: |
Cells on Hydrogels with Micron-Scaled Stiffness Patterns Demonstrate Local Stiffness Sensing |
| المؤلفون: |
Abbas Mgharbel, Camille Migdal, Nicolas Bouchonville, Paul Dupenloup, David Fuard, Eline Lopez-Soler, Caterina Tomba, Marie Courçon, Danielle Gulino-Debrac, Héléne Delanoë-Ayari, Alice Nicolas |
| المصدر: |
Nanomaterials; Volume 12; Issue 4; Pages: 648 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2022 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
stiffness patterning, gray-leveled lithography, traction force microscopy, hydrogel, cell ADHESION, mechanosensitivity |
| الوصف: |
Cell rigidity sensing—a basic cellular process allowing cells to adapt to mechanical cues—involves cell capabilities exerting force on the extracellular environment. In vivo, cells are exposed to multi-scaled heterogeneities in the mechanical properties of the surroundings. Here, we investigate whether cells are able to sense micron-scaled stiffness textures by measuring the forces they transmit to the extracellular matrix. To this end, we propose an efficient photochemistry of polyacrylamide hydrogels to design micron-scale stiffness patterns with kPa/µm gradients. Additionally, we propose an original protocol for the surface coating of adhesion proteins, which allows tuning the surface density from fully coupled to fully independent of the stiffness pattern. This evidences that cells pull on their surroundings by adjusting the level of stress to the micron-scaled stiffness. This conclusion was achieved through improvements in the traction force microscopy technique, e.g., adapting to substrates with a non-uniform stiffness and achieving a submicron resolution thanks to the implementation of a pyramidal optical flow algorithm. These developments provide tools for enhancing the current understanding of the contribution of stiffness alterations in many pathologies, including cancer. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Biology and Medicines; https://dx.doi.org/10.3390/nano12040648 |
| DOI: |
10.3390/nano12040648 |
| الاتاحة: |
https://doi.org/10.3390/nano12040648 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.F8D95413 |
| قاعدة البيانات: |
BASE |