Academic Journal
Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele
| العنوان: | Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele |
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| المؤلفون: | Tomlinson, Laurence, Yang, Ying, Emenecker, Ryan, Smoker, Matthew, Taylor, Jodie, Perkins, Sara, Smith, Justine, MacLean, Dan, Olszewski, Neil E., Jones, Jonathan D. G. |
| سنة النشر: | 2019 |
| المجموعة: | University of East Anglia: UEA Digital Repository |
| الوصف: | The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://ueaeprints.uea.ac.uk/id/eprint/80865/1/pbi.12952.pdf; Tomlinson, Laurence, Yang, Ying, Emenecker, Ryan, Smoker, Matthew, Taylor, Jodie, Perkins, Sara, Smith, Justine, MacLean, Dan, Olszewski, Neil E. and Jones, Jonathan D. G. (2019) Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele. Plant Biotechnology Journal, 17 (1). pp. 132-140. ISSN 1467-7644 |
| DOI: | 10.1111/pbi.12952 |
| الاتاحة: | https://ueaeprints.uea.ac.uk/id/eprint/80865/ https://ueaeprints.uea.ac.uk/id/eprint/80865/1/pbi.12952.pdf https://doi.org/10.1111/pbi.12952 |
| Rights: | cc_by |
| رقم الانضمام: | edsbas.F8D34154 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/pbi.12952 |
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