Academic Journal
Human intestinal epithelial cells promote the differentiation of tolerogenic dendritic cells
| العنوان: | Human intestinal epithelial cells promote the differentiation of tolerogenic dendritic cells |
|---|---|
| المؤلفون: | Iliev, I D, Spadoni, I, Mileti, E, Matteoli, G, Sonzogni, A, Sampietro, G M, Foschi, D, Caprioli, F, Viale, G, Rescigno, M |
| بيانات النشر: | BMJ Publishing Group Ltd |
| سنة النشر: | 2009 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Inflammation |
| الوصف: | Objective: In mice, a subpopulation of gut dendritic cells (DCs) expressing CD103 drives the development of regulatory T (T reg ) cells. Further, it was recently described that the cross-talk between human intestinal epithelial cells (IECs) and DCs helps in maintaining gut immune homeostasis via the induction of non-inflammatory DCs. In this study, an analysis was carried out to determine whether IECs could promote the differentiation of CD103+ tolerogenic DCs, and the function of primary CD103+ DCs isolated from human mesenteric lymph nodes (MLNs) was evaluated. Methods: Monocyte-derived DCs (MoDCs) and circulating CD1c+ DCs were conditioned or not with supernatants from Caco-2 cells or IECs isolated from healthy donors or donors with Crohn’s disease and analysed for their ability to induce T reg cell differentiation. In some cases, transforming growth factor β (TGFβ), retinoic acid (RA) or thymic stromal lymphopoietin (TSLP) were neutralised before conditioning. CD103+ and CD103− DCs were sorted by fluorescence-activated cell sorting (FACS) from MLNs and used in T reg cell differentiation experiments. Results: It was found that human IECs promoted the differentiation of tolerogenic DCs able to drive the development of adaptive Foxp3+ T reg cells. This control was lost in patients with Crohn’s disease and paralleled a reduced expression of tolerogenic factors by primary IECs. MoDCs differentiated with RA or IEC supernatant upregulated the expression of CD103. Consistently, human primary CD103+ DCs isolated from MLNs were endowed with the ability to drive T reg cell differentiation. This subset of DCs expressed CCR7 and probably represents a lamina propria-derived migratory population. Conclusions: A population of tolerogenic CD103+ DCs was identified in the human gut that probably differentiate in response to IEC-derived factors and drive T reg cell development. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://gut.bmj.com/cgi/content/short/58/11/1481; http://dx.doi.org/10.1136/gut.2008.175166 |
| DOI: | 10.1136/gut.2008.175166 |
| الاتاحة: | http://gut.bmj.com/cgi/content/short/58/11/1481 https://doi.org/10.1136/gut.2008.175166 |
| Rights: | Copyright (C) 2009, BMJ Publishing Group |
| رقم الانضمام: | edsbas.F8BD6504 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1136/gut.2008.175166 |
|---|