Academic Journal
Simple HPLC-UV method for therapeutic drug monitoring of 12 antiepileptic drugs and their main metabolites in human plasma
| العنوان: | Simple HPLC-UV method for therapeutic drug monitoring of 12 antiepileptic drugs and their main metabolites in human plasma |
|---|---|
| المؤلفون: | Milosheska, Daniela, Roškar, Robert |
| المصدر: | Molecules, vol. 28, no. 23, 7830, 2023. ; ISSN: 1420-3049 |
| بيانات النشر: | MDPI |
| سنة النشر: | 2023 |
| المجموعة: | University of Ljubljana: Repository (RUJ) / Repozitorij Univerze v Ljubljani |
| مصطلحات موضوعية: | antiepileptic drugs, human plasma, therapeutic drug monitoring, bioanalytical method validation, high-performance liquid chromatography, antiepileptična zdravila, človeška plazma, terapevtsko spremljanje zdravil, validacija bioanalitskih metod, tekočinska kromatografija visoke ločljivosti, epilepsija, info:eu-repo/classification/udc/543.544.5:616.853 |
| الوصف: | The objective of the present report was to develop and validate a simple, selective, and reproducible high-performance liquid chromatography method with UV detection suitable for routine therapeutic drug monitoring of the most commonly used antiepileptic drugs and some of their metabolites. Simple precipitation of plasma proteins with acetonitrile was used for sample preparation. 10,11-dihydrocarbamazepine was used as an internal standard. Chromatographic separation of the analytes was achieved by gradient elution on a Phenyl–Hexyl column at 40 °C, using methanol and potassium phosphate buffer (25 mM ; pH 5.1) as a mobile phase. The method was validated according to the FDA guidelines for bioanalytical method validation. It showed to be selective, accurate, precise, and linear over the concentration ranges of 1–50 mg/L for phenobarbital, phenytoin, levetiracetam, rufinamide, zonisamide, and lacosamide ; 0.5–50 mg/L for lamotrigine, primidone, carbamazepine and 10-monohydroxycarbazepine ; 0.2–10 mg/L for carbamazepine metabolites: 10,11-trans-dihydroxy-10,11-dihydrocarbamazepine and carbamazepine-10,11-epoxide ; 0.1–10 mg/L for oxcarbazepine ; 2–100 mg/L for felbamate and 3–150 mg/L for ethosuximide. The suitability of the validated method for routine therapeutic drug monitoring was confirmed by quantification of the analytes in plasma samples from patients with epilepsy on combination antiepileptic therapy. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf; text/url |
| اللغة: | English |
| Relation: | info:eu-repo/grantAgreement/ARRS//P1-0189; https://repozitorij.uni-lj.si/IzpisGradiva.php?id=152740; https://repozitorij.uni-lj.si/Dokument.php?id=178359&dn=; https://repozitorij.uni-lj.si/Dokument.php?id=178358&dn=; https://plus.cobiss.net/cobiss/si/sl/bib/174900739; http://hdl.handle.net/20.500.12556/RUL-152740 |
| الاتاحة: | https://repozitorij.uni-lj.si/IzpisGradiva.php?id=152740 https://repozitorij.uni-lj.si/Dokument.php?id=178359&dn= https://repozitorij.uni-lj.si/Dokument.php?id=178358&dn= https://plus.cobiss.net/cobiss/si/sl/bib/174900739 https://hdl.handle.net/20.500.12556/RUL-152740 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.F7E1E080 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |