التفاصيل البيبلوغرافية
| العنوان: |
CYTOPROTECTIVE, ANTIOXIDANT AND ANTI‐INFLAMMATORY EFFECTS OF ESSENTIAL OIL AND MAJOR TERPENES OF Satureja macrostema IN ENDOTHELIAL CELLS |
| المؤلفون: |
Torres‐Martínez, Rafael, Hernández‐García, Alejandra, Saavedra‐Molina, Alfredo, López‐Meza, Joel Edmundo, Zarzosa, Alejandra Ochoa, Salgado‐Garciglia, Rafael |
| المساهمون: |
Consejo Nacional de Ciencia y Tecnología |
| المصدر: |
The FASEB Journal ; volume 33, issue S1 ; ISSN 0892-6638 1530-6860 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2019 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Satureja macrostema is a Mexican medicinal plant known as nurite, it is used in decoctions and infusions for the treatment of stomach pain and liver and intestinal diseases, which present inflammatory processes and oxidative stress. In our working group, the in vitro antioxidant activity of the essential oil of aerial parts of S. macrostema obtained by hydrodistillation was determined, and the main volatile terpenes present in this oil were identified (limonene, linalool, menthone, pulegone, thymol and caryophyllene). Therefore, the objetive of the present study was to investigate the cytoprotective, antioxidant and anti‐inflammatory activities of the essential oil of S. macrostema and their major terpenes in bovine umbilical vein endothelial cells (BUVEC), under oxidative stress induced by H 2 O 2 and an inflammatory state induced by lipopolysaccharide (LPS). Cell viability was evaluated by the MTT reduction assay and trypan blue exclusion. BUVEC cells were incubated with the essential oil and the major terpenes (limonene, linalool, menthone, pulegone, thymol and caryophyllene) of S. macrostema at concentrations of 1, 10 100 and 1000 μg/mL; finding that pretreatment of BUVEC with essential oil of S. macrostema and the terpenes limonene, linalool, menthone, pulegone, thymol and caryophyllene showed no cytotoxicity at concentrations of 1, 10 and 100 μg/mL; with a viability greater than 85% of the epithelial bovine cells. The production of reactive species of oxygen (ROS) and nitrogen (RNS) were measured by Flow cytometry in BUVEC cells incubated with H 2 O 2 2% during 24 h after pretreatment with the essential oil and terpenes of S. macrostema (1, 10 and 100 μg/mL). Production of cytoplasmic and mitochondrial ROS, nitric oxide, superoxide anion and hydroxyl radical in BUVEC treated with H 2 O 2 decreased with the essential oil and the six major terpenes at the three concentrations tested. Quantification of the gene expression of TNF‐α and IL‐10 cytokine was carried out by real‐time qPCR inducing inflammation with ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1096/fasebj.2019.33.1_supplement.471.22 |
| الاتاحة: |
http://dx.doi.org/10.1096/fasebj.2019.33.1_supplement.471.22 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: |
edsbas.F7634A1F |
| قاعدة البيانات: |
BASE |