Academic Journal
Human chorionic gonadotropin with C-elongated alpha-subunit retains full receptor binding and partial agonist activity
| العنوان: | Human chorionic gonadotropin with C-elongated alpha-subunit retains full receptor binding and partial agonist activity |
|---|---|
| المؤلفون: | Marichatou, H, Martinat, N, Guillou, F, Combarnous, Y |
| المصدر: | European Journal of Endocrinology ; page 402-405 ; ISSN 0804-4643 1479-683X |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Endocrinology, General Medicine, Endocrinology, Diabetes and Metabolism |
| الوصف: | OBJECTIVE: To test whether extension of the C-terminus of human chorionic gonadotropin (hCG) alpha-subunit (halpha) alters the bioactivity of the recombined alphabeta heterodimer. DESIGN: The stop codon of halpha was mutated to produce a 24 amino acid extension. METHODS: The extended halpha (alpha(+24)) was co-expressed with hCGbeta in COS-7 cells and the receptor binding and in vivo bioactivity of the secreted hormone was compared with its wild-type counterpart. RESULTS: This extension did not impair the binding of hCG to rat LH/CG receptors and provoked a sixfold reduction in its stimulatory activity of testosterone secretion in rat Leydig cells. CONCLUSIONS: The extension of alpha by itself does not lead to inhibition of the alphabeta heterodimer to LH receptors but the structure of the extension appears to play an important role. It is thus possible that one-chain hCG chimeras with the beta N-terminus fused to the alpha C-terminus might be active. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.1530/eje.0.1420402 |
| الاتاحة: | http://dx.doi.org/10.1530/eje.0.1420402 https://eje.bioscientifica.com/view/journals/eje/142/4/402.xml |
| رقم الانضمام: | edsbas.F6FC893D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1530/eje.0.1420402 |
|---|