التفاصيل البيبلوغرافية
| العنوان: |
Cardiac-specific p38γ/δ act overexpression decreases cardiac glycogen storage and lipid accumulation. |
| المؤلفون: |
Ayelén M. Santamans (5487941), Valle Montalvo-Romeral (11675607), Alfonso Mora (335370), Juan Antonio Lopez (11675610), Francisco González-Romero (11675613), Daniel Jimenez-Blasco (11675616), Elena Rodríguez (2513533), Aránzazu Pintor-Chocano (11675619), Cristina Casanueva-Benítez (11675622), Rebeca Acín-Pérez (106137), Luis Leiva-Vega (5487962), Jordi Duran (36345), Joan J. Guinovart (11675625), Jesús Jiménez-Borreguero (11675628), José Antonio Enríquez (9446609), María Villlalba-Orero (11675631), Juan P. Bolaños (7953083), Patricia Aspichueta (9178163), Jesús Vázquez (54003), Bárbara González-Terán (11675634), Guadalupe Sabio (5487935) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Cell Biology, Genetics, Molecular Biology, Physiology, Pharmacology, Developmental Biology, Cancer, Virology, Computational Biology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Information Systems not elsewhere classified, p38 &# 948, p38 &# 947, maladaptive cardiac pathogenesis, glycolytic energy production, glycogen synthase 1, glycogen metabolism inactivation, cardiomyocyte fuel supply, cardiac glycogen production, activated protein kinases, early metabolic shift, body metabolic deregulation, maternal diet supplementation, fatty acid oxidation, postnatal heart development, newborn mice results, early gys1 phosphorylation, metabolic change |
| الوصف: |
Mice were IV injected at PD1 with AAV-cTnT-GFP-Luc (TnTGFP) or AAV-cTnT-p38γ/δ act (TnTp38γ/δ act ) and killed at PD14. (A) PAS staining in heart sections of TnTp38γ/δ act or TnTGFP mice (left) and quantification (right). Scale bar: 200 μm . (B) Cardiac glycogen quantification. (C) Conversion of [3- 3 H] glucose into 3 H 2 O, reflecting the glycolytic rate in hearts from TnTGFP and TnTp38γ/δ act mice. (D) qRT-PCR analysis of glycolytic enzymes expression in cardiac tissue. (E) ORO staining in heart sections, showing representative images. Quantification chart below. Scale bar: 50 μm . (F) Cardiac fatty acid oxidation rate of 14 C-palmitate determined by the production of CO 2 and ASMs. (G) Cardiac lipid profile. All lipid amounts were normalized by mg of protein except for NEFAs, which were relativized by mg of tissue. Data are mean ± SEM. ( n = 5–12). * p < 0.05; ** p < 0.01; *** p < 0.001 by Student t test. Raw data are given in S14 Fig . ASM, acid-soluble metabolite; CL, cardiolipin; DG, diglycerides; FC, free cholesterol; LPC, lysophosphatidylcholine; NEFA, non-esterified fatty acid; ORO, oil red O; PAS, periodic acid–Schiff; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; qRT-PCR, real-time quantitative PCR; TG, triglyceride. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Cardiac-specific_p38_sup_act_sup_overexpression_decreases_cardiac_glycogen_storage_and_increases_lipid_accumulation_/16984476 |
| DOI: |
10.1371/journal.pbio.3001447.g002 |
| الاتاحة: |
https://doi.org/10.1371/journal.pbio.3001447.g002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.F6F6A10C |
| قاعدة البيانات: |
BASE |