Academic Journal
Deletion of Ck2β gene causes germ cell development arrest and azoospermia in male mice
| العنوان: | Deletion of Ck2β gene causes germ cell development arrest and azoospermia in male mice |
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| المؤلفون: | Liang, Qiu‐Xia, Wang, Zhen‐Bo, Lei, Wen‐Long, Lin, Fei, Qiao, Jing‐Yi, Filhol‐Cochet, Odile, Boldyreff, Brigitte, Schatten, Heide, Sun, Qing‐Yuan, Qian, Wei‐Ping |
| المساهمون: | National Aerospace Science Foundation of China, Youth Innovation Promotion Association of the Chinese Academy of Sciences |
| المصدر: | Cell Proliferation ; volume 53, issue 1 ; ISSN 0960-7722 1365-2184 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2019 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Objectives In humans, non‐obstructive azoospermia (NOA) is a major cause of male infertility. However, the aetiology of NOA is largely unknown. Previous studies reported that protein CK2β was abundantly and broadly expressed in spermatogenic cells. Here, we investigate whether protein CK2β participates in spermatogenesis. Materials and Methods In this study, we separated spermatogenic cells using STA‐PUT velocity sedimentation, analysed the expression pattern of protein CK2β by immunoblotting, specifically deleted Ck2β gene in early‐stage spermatogenic cells by crossing Ck2β fl mice with Stra8‐Cre + mice and validated the knockout efficiency by quantitative RT‐PCR and immunoblotting. The phenotypes of Ck2β fl/Δ ;SCre + mice were studied by immunohistochemistry and immunofluorescence. The molecular mechanisms of male germ cell development arrest were elucidated by immunoblotting and TUNEL assay. Results Ablation of Ck2β gene triggered excessive germ cell apoptosis, germ cell development arrest, azoospermia and male infertility. Inactivation of Ck2β gene caused distinctly reduced expression of Ck2α ′ gene and CK2α′ protein. Conclusions Ck2β is a vital gene for germ cell survival and male fertility in mice. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/cpr.12726 |
| الاتاحة: | http://dx.doi.org/10.1111/cpr.12726 https://onlinelibrary.wiley.com/doi/pdf/10.1111/cpr.12726 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/cpr.12726 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.F6F4443A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/cpr.12726 |
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