Academic Journal
NDK-1, the Homolog of NM23-H1/H2 Regulates Cell Migration and Apoptotic Engulfment in C. elegans.
| العنوان: | NDK-1, the Homolog of NM23-H1/H2 Regulates Cell Migration and Apoptotic Engulfment in C. elegans. |
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| المؤلفون: | Fancsalszky, L, Monostori, E, Farkas, Z, Pourkarimi, E, Masoudi, N, Hargitai, B, Bosnar, MH, Dezeljin, M, Zsakai, A, Vellai, Tibor, Mehta, A, Takacs-Vellai, K |
| سنة النشر: | 2014 |
| المجموعة: | Eötvös Loránd University: ELTE Digital Institutional Repository (EDIT) / Eötvös Loránd Tudományegyetem |
| الوصف: | Abnormal regulation of cell migration and altered rearrangement of cytoskeleton are characteristic of metastatic cells. The first described suppressor of metastatic processes is NM23-H1, which displays NDPK (nucleoside-diphosphate kinase) activity. To better understand the role of nm23 genes in cell migration, we investigated the function of NDK-1, the sole Caenorhabditis elegans homolog of group I NDPKs in distal tip cell (DTC) migration. Dorsal phase of DTC migration is regulated by integrin mediated signaling. We find that ndk-1 loss of function mutants show defects in this phase. Epistasis analysis using mutants of the alpha-integrin ina-1 and the downstream functioning motility-promoting signaling module (referred to as CED-10 pathway) placed NDK-1 downstream of CED-10/Rac. As DTC migration and engulfment of apoptotic corpses are analogous processes, both partially regulated by the CED-10 pathway, we investigated defects of apoptosis in ndk-1 mutants. Embryos and germ cells defective for NDK-1 showed an accumulation of apoptotic cell corpses. Furthermore, NDK-1::GFP is expressed in gonadal sheath cells, specialized cells for engulfment and clearence of apoptotic corpses in germ line, which indicates a role for NDK-1 in apoptotic corpse removal. In addition to the CED-10 pathway, engulfment in the worm is also mediated by the CED-1 pathway. abl-1/Abl and abi-1/Abi, which function in parallel to both CED-10/CED-1 pathways, also regulate engulfment and DTC migration. ndk-1(-);abi-1(-) double mutant embryos display an additive phenotype (e. g. enhanced number of apoptotic corpses) which suggests that ndk-1 acts in parallel to abi-1. Corpse number in ndk-1(-);ced-10(-) double mutants, however, is similar to ced-10(-) single mutants, suggesting that ndk-1 acts downstream of ced-10 during engulfment. In addition, NDK-1 shows a genetic interaction with DYN-1/dynamin, a downstream component of the CED-1 pathway. In summary, we propose that NDK-1/NDPK might represent a converging point of CED-10 and CED-1 pathways in ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | http://hdl.handle.net/10831/66778; elte:000333355300113; elte:84899109859; elte:2564381; elte:3; elte:PLOS ONE; elte:9; elte:24658123; elte:10082784; LOMS: https://edit.elte.hu/xmlui/bitstream/10831/66778/1/2564381.pdf |
| DOI: | 10.1371/journal.pone.0092687 |
| الاتاحة: | http://hdl.handle.net/10831/66778 https://doi.org/10.1371/journal.pone.0092687 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.F6C0410B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0092687 |
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