التفاصيل البيبلوغرافية
| العنوان: |
Transfection of the cloned human excision repair gene ERCC-1 to UV-sensitive CHO mutants only corrects the repair defect in complementation group 2 mutants. |
| المؤلفون: |
Duin, M. (Mark) van, Janssen, J.H., Wit, J. (Jan) de, Hoeijmakers, J.H.J. (Jan), Thompson, L.H. (Larry), Bootsma, D. (Dirk), Westerveld, A. (Andries) |
| المصدر: |
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis vol. 193, pp. 123-130 |
| سنة النشر: |
1988 |
| المجموعة: |
RePub - Publications from Erasmus University, Rotterdam |
| مصطلحات موضوعية: |
DNA Repair, Genes, Structural/radiation effects, Genetic Complementation Test, Mutation, Transfection, 0 (Cosmids), Animals, Cell Line, Cloning, Molecular, Cosmids, Cricetulus, DNA Replication/radiation effects, Hamsters, Human, Support, Non-U.S. Gov't, U.S. Gov't, Non-P.H.S, Ultraviolet Rays |
| الوصف: |
The human DNA-excision repair gene ERCC-1 is cloned by its ability to correct the excision-repair defect of the ultraviolet light- and mitomycin-C-sensitive CHO mutant cell line 43-3B. This mutant is assigned to complementation group 2 of the excision-repair-deficient CHO mutants. In order to establish whether the correction by ERCC-1 is confined to CHO mutants of one complementation group, the cloned repair gene, present on cosmid 43-34, was transfected to representative cell lines of the 6 complementation groups that have been identified to date. Following transfection, mycophenolic acid was used to select for transferants expressing the dominant marker gene Ecogpt, also present on cosmid 43-34. Cotransfer of the ERCC-1 gene was shown by |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
http://repub.eur.nl/pub/2998; urn:hdl:1765/2998 |
| الاتاحة: |
http://repub.eur.nl/pub/2998 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.F668C4C7 |
| قاعدة البيانات: |
BASE |