Academic Journal
Reproductive Failure Experimentally Induced in Sows via Artificial Insemination with Semen Spiked with Porcine Circovirus Type 2
| العنوان: | Reproductive Failure Experimentally Induced in Sows via Artificial Insemination with Semen Spiked with Porcine Circovirus Type 2 |
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| المؤلفون: | Madson, D. M., Patterson, A. R., Ramamoorthy, S., Pal, N., Meng, X. J., Opriessnig, T. |
| المصدر: | Veterinary Pathology ; volume 46, issue 4, page 707-716 ; ISSN 0300-9858 1544-2217 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2009 |
| الوصف: | Porcine circovirus type 2 (PCV2) is associated with reproductive failure in female pigs. However, the association of PCV2-positive semen in the pathogenesis has not been elucidated. The objectives of this study were to determine whether semen spiked with PCV2 causes infection in PCV2-naïve, mature female pigs and whether delivery of PCV2 via artificial insemination causes reproductive failure or fetal infection. Nine sows were randomly allocated into 3 groups of 3 sows each and artificially inseminated with PCV2 DNA-negative semen (group 1), PCV2 DNA-negative semen spiked with PCV2a (group 2), or PCV2b (group 3). All sows in groups 2 and 3 developed PCV2 viremia 7 to 14 days after insemination. None of the group 2 sows became pregnant, whereas all group 3 sows (3/3) farrowed at the expected date. At parturition, presuckle serum samples were collected, and live-born piglets, stillborn fetuses, and mummified fetuses were necropsied. All live-born piglets ( n = 8) in group 3 were PCV2 viremic at birth. Stillborn fetuses ( n = 2) had gross lesions of congestive heart failure. Mummified fetuses ( n = 25) varied in crown-rump length from 7 to 27 cm, indicating fetal death between 42 and 105 days of gestation. PCV2 antigen was detected in the myocardium by immunohistochemistry of 7/8 (88%) live-born piglets, 2/2 (100%) of the stillborn fetuses, and 25/25 (100%) of the mummified fetuses. In addition, 4/25 mummified fetuses had PCV2 antigen associated with smooth muscle cells and fibroblasts. The results of this study indicate that intrauterine administration of PCV2 causes reproductive failure in naïve sows. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1354/vp.08-vp-0234-o-fl |
| DOI: | 10.1354/vp.08-VP-0234-O-FL |
| الاتاحة: | https://doi.org/10.1354/vp.08-vp-0234-o-fl https://journals.sagepub.com/doi/pdf/10.1354/vp.08-VP-0234-O-FL https://journals.sagepub.com/doi/full-xml/10.1354/vp.08-VP-0234-O-FL |
| Rights: | https://journals.sagepub.com/page/policies/text-and-data-mining-license |
| رقم الانضمام: | edsbas.F5E89DFF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1354/vp.08-vp-0234-o-fl |
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