التفاصيل البيبلوغرافية
| العنوان: |
USE OF COMBINATION OF FLUORESCENT PROBES TO IDENTIFY SPERM SUBPOPULATIONS FOR QUALITY ASSESSMENT OF FRESH AND CRYOPRESERVED CANINE SEMEN. PRELIMINARY RESULTS |
| المؤلفون: |
Csermak Jr, Antonio Carlos, de Paula, Tarcízio A. R., Da Costa, Eduardo Paulino, Guimarães, José Domingos, Bergo C.F, Letícia, Ribeiro de Araujo, Gediendson, De Deco-Souza, Thyara, De Morais Garay, Rafael |
| المصدر: |
JOURNAL OF VETERINARY ANDROLOGY; Vol. 3, Núm. 1 (2018): ENERO-JUNIO / JANUARY-JUNE ; 2542-3045 |
| بيانات النشر: |
CENTRO DE ESTUDIOS SUPERIORES E INVESTIGACIÓN EN CIENCIAS DEL AGRO (CESICA) |
| سنة النشر: |
2018 |
| المجموعة: |
Journal of Veterinary Andrology |
| مصطلحات موضوعية: |
semen canino, yoduro de propidio, Hoechst 33342, FITC-PSA, criopreservación |
| الوصف: |
The use of fluorescent markers in the evaluation of sperm morphophysiology allows a better accuracy, compared to the subjective nature of some routine tests in semen qualification. In this study was used the combination of fluorescence probes: propionate iodide, Hoechst 33342 and FITC-PSA in fresh and thawed dog semen, to the identification of the following morphological subpopulations: II (intact plasma and acrosomal membranes), IL (intact plasma membrane and lesioned acrosomal membrane), LI (lesioned plasma membrane and intact acrosomal membrane) and LL (both membranes lesioned). When comparing the results obtained with the results of the tests used conventionally in semen evaluation (sperm motility and vigor, hypoosmotic test and morphological alterations), little correlation was observed. The II population declined from fresh semen to thawed, while LL population increased (p <0.05). The IL population was composed of extremely small numbers of cells but increased (p <0.05) from fresh semen to thawed semen. In the thawed semen the major defects had a positive correlation with the LL population (p <0.01). For the thawed semen, the results of the hypoosmotic test (number of cells that reacted to the medium) correlated positively with population II (p <0.025), that is, different from that observed in fresh semen. Although all tests were able to detect decrease in sperm quality post-thawing (p <0.05). The use of this fluorescent probe association allowed qualification and more accurately quantification of plasma membrane and acrosomal insults mediated by cryopreservation. El uso de marcadores fluorescentes en la evaluación de la morfofisiología espermática permite una mayor precisión, comparada con la naturaleza subjetiva de algunas pruebas de rutina en la valoración del semen. En este estudio se usó la combinación de pruebas fluorescentes: yoduro de propidio; Hoechst 33342 y FITC-PSA en semen fresco y descongelado de perro, para la identificación de las siguientes subpoblaciones morfológicas: II ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
Spanish; Castilian |
| Relation: |
http://cesica.org/publicaciones/index.php/journal_veterinary_andrology/article/view/43/37; http://cesica.org/publicaciones/index.php/journal_veterinary_andrology/article/view/43 |
| الاتاحة: |
http://cesica.org/publicaciones/index.php/journal_veterinary_andrology/article/view/43 |
| Rights: |
Copyright (c) 2018 JOURNAL OF VETERINARY ANDROLOGY ; https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.F5A4A009 |
| قاعدة البيانات: |
BASE |