Academic Journal
Comparison of the sensitivities of crustacean species to recombinant Photorhabdus insect-related (Pir) toxins of acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus
| العنوان: | Comparison of the sensitivities of crustacean species to recombinant Photorhabdus insect-related (Pir) toxins of acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus |
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| المؤلفون: | Kim, Ji Seok, Jang, In-Kwon, Lee, Miyoung, Kim, Suhyun, Uhm, Taewoong, Yi, Seongbaek, Choi, Tae-Jin |
| المصدر: | Crustaceana ; volume 95, issue 7, page 827-843 ; ISSN 0011-216X 1568-5403 |
| بيانات النشر: | Brill |
| سنة النشر: | 2022 |
| الوصف: | Photorhabdus insect-related PirA vp (where vp stands for Vibrio parahaemolyticus ) and PirB vp of Vibrio parahaemolyticus were expressed in Escherichia coli , designated rPirA vp and rPirB vp , respectively, then tested on 4 crustaceans via immersion tests. PirB vp toxin was detected from 2 h after incubation and accumulated with bacterial growth. The culture filtrate of 15 nM PirB vp toxin caused 100% mortality of Litopenaeus vannamei within 4 days after immersion. Litopenaeus vannamei showed 100% mortality at 7 days after immersion in purified 4 nM rPirA vp , rPirB vp , or rPirA vp + rPirB vp mixture. The cumulative mortalities in the rPirA vp , rPirB vp or rPirA vp + rPirB vp mixture were 50, 83 and 100% in Macrobrachium rosenbergii after 12 days of immersion, 11, 77 and 100% in Palaemon paucidens after 10 days of immersion, and 36, 96 and 100% in Macrophthalmus japonicus after 6 days of immersion, respectively. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.1163/15685403-bja10230 |
| الاتاحة: | http://dx.doi.org/10.1163/15685403-bja10230 https://brill.com/view/journals/cr/95/7/article-p827_8.xml https://brill.com/downloadpdf/journals/cr/95/7/article-p827_8.xml |
| رقم الانضمام: | edsbas.F58CE407 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1163/15685403-bja10230 |
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