Academic Journal
A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections
| العنوان: | A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections |
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| المؤلفون: | Wang, G, Glaser, L, Scott, NE, Fathy Mohamed, Y, Ingram, R, Laroucau, K, Valvano, MA |
| بيانات النشر: | TAYLOR & FRANCIS INC |
| سنة النشر: | 2021 |
| المجموعة: | The University of Melbourne: Digital Repository |
| الوصف: | We recently described a protein O-glycosylation pathway conserved in all species of the Burkholderia genus that results in the synthesis and incorporation of a trisaccharide glycan to membrane-exported proteins. Here, we exploited this system to construct and evaluate a diagnostic tool for glanders. Burkholderia mallei, the causative agent of glanders, is a highly infectious and fatal zoonotic pathogen that infects horses, mules, donkeys, and occasionally humans. A highly sensitive and specific diagnostic tool is crucial for the control, elimination, and eradication of B. mallei infections. We constructed plasmids carrying synthetic genes encoding a modified, previously unannotated Burkholderia glycoprotein containing three glycosylation sequons fused to the cholera toxin B-subunit. The resulting proteins were glycosylated in the B. cenocepacia K56-2 parental strain, but not in glycosylation-deficient mutants, as determined by SDS-PAGE and fluorescent lectin blots. One of these glycoproteins was used as an antigen in ELISA and western blots to screen a panel of serum samples collected from glanders-infected and healthy horses, which were previously investigated by complement fixation test and indirect ELISA based on a semi-purified fraction of B. mallei. We show that ELISA and western blot assays based on our glycoprotein antigen provide 100% specificity, with a sensitivity greater than 88%. The glycoprotein antigen was recognized by serum samples collected from patients infected with B. pseudomallei, B. mallei, B. multivorans, and B. cenocepacia. Our results indicate that protein O-glycosylation in Burkholderia can be exploited as a biomarker for diagnosis of Burkholderia-associated infections. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2150-5594 2150-5608 |
| Relation: | NHMRC/1100164; Wang, G., Glaser, L., Scott, N. E., Fathy Mohamed, Y., Ingram, R., Laroucau, K. & Valvano, M. A. (2021). A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections. VIRULENCE, 12 (1), pp.493-506. https://doi.org/10.1080/21505594.2021.1876440.; http://hdl.handle.net/11343/272846 |
| الاتاحة: | http://hdl.handle.net/11343/272846 |
| Rights: | CC BY ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.F555225B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 21505594 21505608 |
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