Academic Journal
Evidence that Ubiquinone Is a Required Intermediate for Rhodoquinone Biosynthesis in Rhodospirillum rubrum
| العنوان: | Evidence that Ubiquinone Is a Required Intermediate for Rhodoquinone Biosynthesis in Rhodospirillum rubrum |
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| المؤلفون: | Brajcich, Brian C., Iarocci, Andrew L., Johnstone, Lindsey A. G., Morgan, Rory K., Lonjers, Zachary T., Hotchko, Matthew J., Muhs, Jordan D., Kieffer, Amanda, Reynolds, Bree J., Mandel, Sarah M., Marbois, Beth N., Clarke, Catherine F., Shepherd, Jennifer N. |
| المصدر: | Journal of Bacteriology ; volume 192, issue 2, page 436-445 ; ISSN 0021-9193 1098-5530 |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2010 |
| الوصف: | Rhodoquinone (RQ) is an important cofactor used in the anaerobic energy metabolism of Rhodospirillum rubrum . RQ is structurally similar to ubiquinone (coenzyme Q or Q), a polyprenylated benzoquinone used in the aerobic respiratory chain. RQ is also found in several eukaryotic species that utilize a fumarate reductase pathway for anaerobic respiration, an important example being the parasitic helminths. RQ is not found in humans or other mammals, and therefore inhibition of its biosynthesis may provide a parasite-specific drug target. In this report, we describe several in vivo feeding experiments with R. rubrum used for the identification of RQ biosynthetic intermediates. Cultures of R. rubrum were grown in the presence of synthetic analogs of ubiquinone and the known Q biosynthetic precursors demethylubiquinone, demethoxyubiquinone, and demethyldemethoxyubiquinone, and assays were monitored for the formation of RQ 3 . Data from time course experiments and S -adenosyl- l -methionine-dependent O -methyltransferase inhibition studies are discussed. Based on the results presented, we have demonstrated that Q is a required intermediate for the biosynthesis of RQ in R. rubrum . |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/jb.01040-09 |
| DOI: | 10.1128/JB.01040-09 |
| الاتاحة: | http://dx.doi.org/10.1128/jb.01040-09 https://journals.asm.org/doi/pdf/10.1128/JB.01040-09 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.F52E2484 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/jb.01040-09 |
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