التفاصيل البيبلوغرافية
| العنوان: |
Core-EJC depletion yields broad activation of de novo splice junctions. |
| المؤلفون: |
Brian Joseph (10873295), Eric C. Lai (10535917) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Cell Biology, Genetics, Molecular Biology, Evolutionary Biology, Developmental Biology, Hematology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Mathematical Sciences not elsewhere classified, Exon Junction Complex, EJC regulation triggers, splice sites, splice site selection, SS |
| الوصف: |
(A) Strong overlap of de novo splice junctions between core-EJC knockdown conditions. The Venn diagram depicts which of 1677 junctions with at least 5 split reads had > 2-fold split read changes between treatment and controls. p-value for three-way overlap was calculated using a permutation test with 10^8 tests. (B) Strong overlap of high-confidence de novo splice junctions between core-EJC knockdown conditions. The Venn diagram depicts which of 876 junctions with at least 5 split reads and > 2-fold split read changes also show > 2-fold changes in percent selected index (PSI) between treatment and controls. p -value for three-way overlap was calculated using a permutation test with 10^8 tests. (C) Knockdown of EJC factors in S2 cells using dsRNA. quantitative rt-PCR of core-EJC and btz transcripts after dsRNA treatment. (D) Sashimi plot illustrating the expression of annotated alternative isoforms for unkempt (unk) in S2 cells. The location of rtPCR primers and alternative isoforms are included. (TIF) |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Core-EJC_depletion_yields_broad_activation_of_i_de_novo_i_splice_junctions_/14675243 |
| DOI: |
10.1371/journal.pgen.1009563.s001 |
| الاتاحة: |
https://doi.org/10.1371/journal.pgen.1009563.s001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.F51929BA |
| قاعدة البيانات: |
BASE |