Academic Journal
Inositol Phosphates Purification Using Titanium Dioxide Beads
| العنوان: | Inositol Phosphates Purification Using Titanium Dioxide Beads |
|---|---|
| المؤلفون: | Wilson, MS, Saiardi, A |
| المصدر: | Bio-Protocol , 8 (15) , Article 2959. (2018) |
| سنة النشر: | 2018 |
| المجموعة: | University College London: UCL Discovery |
| مصطلحات موضوعية: | Inositol polyphosphate, Inositol pyrophosphate, IP₆, IP₇, Metabolism, Metabolites, Signaling |
| الوصف: | Inositol phosphates (IPs) comprise a family of ubiquitous eukaryotic signaling molecules. They have been linked to the regulation of a pleiotropy of important cellular activities, but low abundance and detection difficulties have hampered our understanding. Here we present a method to purify and enrich IPs or other phosphate-rich metabolites from mammalian cells or other sample types. Acid-extracted IPs from cells bind selectively via their phosphate groups to titanium dioxide beads. After washing, the IPs are easily eluted from the beads by increasing the pH. This technique, in combination with downstream analytical methods such as PAGE or SAX-HPLC, opens unprecedented investigative possibilities, allowing appropriate analysis of IPs from virtually any biological or non-biological source. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | https://discovery.ucl.ac.uk/id/eprint/10057343/1/Wilson_Inositol%20Phosphates%20Purification%20Using%20Titanium%20Dioxide%20Beads_VoR.pdf; https://discovery.ucl.ac.uk/id/eprint/10057343/ |
| الاتاحة: | https://discovery.ucl.ac.uk/id/eprint/10057343/1/Wilson_Inositol%20Phosphates%20Purification%20Using%20Titanium%20Dioxide%20Beads_VoR.pdf https://discovery.ucl.ac.uk/id/eprint/10057343/ |
| Rights: | open |
| رقم الانضمام: | edsbas.F4C92A85 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |