التفاصيل البيبلوغرافية
| العنوان: |
Four state model for efficient simulation of gap junction channel voltage gating kinetics |
| المؤلفون: |
Snipas, M., Kraujalis, T., Maciunas, K., Rimkute, L., Gudaitis, L., Verselis, V. |
| المصدر: |
European biophysics journal: with biophysics letters: Joint 12th EBSA congress and 10th ICBP – IUPAP congress: July 20‐24, 2019, Madrid, Spain, New York, NY : Springer, 2019, vol. 48, suppl. 1, P-156, p. 118 ; ISSN 0175-7571 ; eISSN 1432-1017 |
| سنة النشر: |
2019 |
| المجموعة: |
LSRC VL (Lithuanian Social Research Centre Virtual Library) / LSTC VB (Lietuvos socialinių tyrimų centras virtualią biblioteką) |
| مصطلحات موضوعية: |
gap junction (GJ) channels, connexin (Cx) proteins, kinetics |
| الوصف: |
Gap junction (GJ) channels, formed of connexin (Cx) proteins, provide a direct pathway for metabolic and electrical cell-to-cell communication. These specialized channels are not just passive conduits for the passage of ions and metabolites, but have been shown to gate robustly in response to transjunctional voltage, Vj, the voltage difference between coupled cells and are regulated by various chemical factors. Voltage gating of GJs may play a physiological role, particularly in excitable cells which can exhibit large transients in membrane potential during the generation of an action potential. In this paper, we present a mathematical/computational model of GJ channel voltage gating based on assumption that two apposing hemichannels are connected in series, and activation energies for their gating transitions between an open and residual state linearly depend on voltage across each hemichannel. To demonstrate the viability of the model, we fit experimental data obtained from electrophysiological recordings in cell cultures transfected with Cx43 and Cx45, isoforms expressed in cardiac tissue. The results show that the model is capable of describing both steady-state and kinetic properties of homotypic and heterotypic GJ channels. Moreover, mathematical analyses showed that the model can be simplified to a reversible two-state system and solved analytically, using a rapid equilibrium assumption. Given that excitable cells are arranged in interconnected networks, the equilibrium assumption allows for a substantial reduction in computation time, which is useful in simulations of large cell clusters. Overall, this model can serve not just as a modeling tool, but also to provide a means of testing GJ channel gating behavior. |
| نوع الوثيقة: |
conference object |
| اللغة: |
English |
| Relation: |
http://ktu.lvb.lt/KTU:ELABAPDB39966749&prefLang=en_US |
| الاتاحة: |
http://ktu.lvb.lt/KTU:ELABAPDB39966749&prefLang=en_US |
| رقم الانضمام: |
edsbas.F4C5A803 |
| قاعدة البيانات: |
BASE |