التفاصيل البيبلوغرافية
| العنوان: |
Drosophila Models Rediscovered with Super-Resolution Microscopy |
| المؤلفون: |
Szilárd Szikora, Péter Görög, Csaba Kozma, József Mihály |
| المصدر: |
Cells; Volume 10; Issue 8; Pages: 1924 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2021 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
nanoscopy, super-resolution microscopy, SIM, STED, SMLM, Drosophila, active zone, centrosome, sarcomere |
| الوصف: |
With the advent of super-resolution microscopy, we gained a powerful toolbox to bridge the gap between the cellular- and molecular-level analysis of living organisms. Although nanoscopy is broadly applicable, classical model organisms, such as fruit flies, worms and mice, remained the leading subjects because combining the strength of sophisticated genetics, biochemistry and electrophysiology with the unparalleled resolution provided by super-resolution imaging appears as one of the most efficient approaches to understanding the basic cell biological questions and the molecular complexity of life. Here, we summarize the major nanoscopic techniques and illustrate how these approaches were used in Drosophila model systems to revisit a series of well-known cell biological phenomena. These investigations clearly demonstrate that instead of simply achieving an improvement in image quality, nanoscopy goes far beyond with its immense potential to discover novel structural and mechanistic aspects. With the examples of synaptic active zones, centrosomes and sarcomeres, we will explain the instrumental role of super-resolution imaging pioneered in Drosophila in understanding fundamental subcellular constituents. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Cell and Gene Therapy; https://dx.doi.org/10.3390/cells10081924 |
| DOI: |
10.3390/cells10081924 |
| الاتاحة: |
https://doi.org/10.3390/cells10081924 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.F4679139 |
| قاعدة البيانات: |
BASE |