Academic Journal
Comparison of BinaxNOW and SARS-CoV-2 qRT-PCR detection of the omicron variant from matched anterior nares swabs
| العنوان: | Comparison of BinaxNOW and SARS-CoV-2 qRT-PCR detection of the omicron variant from matched anterior nares swabs |
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| المؤلفون: | Landaverde, Lena, Turcinovic, Jacquelyn, Doucette-Stamm, Lynn, Gonzales, Kevin, Platt, Judy, Connor, John H., Klapperich, Catherine |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2023 |
| المجموعة: | Boston University: OpenBU |
| مصطلحات موضوعية: | BinaxNow, COVID-19, Omicron, RT-PCR, SARS-CoV-2, Anterior nares, Antigen, Rapid diagnostic tests, Humans, Pandemics, Reverse transcription, Polymerase chain reaction |
| جغرافية الموضوع: | United States |
| الوصف: | The COVID-19 pandemic has increased use of rapid diagnostic tests (RDTs). In winter 2021 to 2022, the Omicron variant surge made it apparent that although RDTs are less sensitive than quantitative reverse transcription-PCR (qRT-PCR), the accessibility, ease of use, and rapid readouts made them a sought after and often sold-out item at local suppliers. Here, we sought to qualify the Abbott BinaxNOW RDT for use in our university testing program as a method to rule in positive or rule out negative individuals quickly at our priority qRT-PCR testing site. To perform this qualification study, we collected additional swabs from individuals attending this site. All swabs were tested using BinaxNOW. Initially as part of a feasibility study, test period 1 (n = 110) samples were stored cold before testing. In test period 2 (n = 209), samples were tested immediately. Combined, 102/319 samples tested severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) positive via qRT-PCR. All sequenced samples were Omicron (n = 92). We calculated 53.9% sensitivity, 100% specificity, a 100% positive predictive value, and an 82.2% negative predictive value for BinaxNOW (n = 319). Sensitivity would be improved (75.3%) by changing the qRT-PCR positivity threshold from a threshold cycle (CT) value of 40 to a CT value of 30. The receiver operating characteristic (ROC) curve shows that for qRT-PCR-positive CT values of between 24 and 40, the BinaxNOW test is of limited value diagnostically. Results suggest BinaxNOW could be used in our setting to confirm SARS-CoV-2 infection in individuals with substantial viral load, but a significant fraction of infected individuals would be missed if we used RDTs exclusively to rule out infection. IMPORTANCE Our results suggest BinaxNOW can rule in SARS-CoV-2 infection but would miss infections if RDTs were exclusively used. ; Boston University ; Published version |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | p. e0130722; Print-Electronic |
| اللغة: | English |
| تدمد: | 2165-0497 |
| Relation: | Microbiology Spectrum; https://www.ncbi.nlm.nih.gov/pubmed/36255297; L. Landaverde, J. Turcinovic, L. Doucette-Stamm, K. Gonzales, J. Platt, J.H. Connor, C. Klapperich. 2022. "Comparison of BinaxNOW and SARS-CoV-2 qRT-PCR Detection of the Omicron Variant from Matched Anterior Nares Swabs." Microbiology Spectrum, Volume 10, Issue 6, pp.e0130722-. https://doi.org/10.1128/spectrum.01307-22; https://hdl.handle.net/2144/45833; 0000-0002-8867-7256 (Connor, John H); 0000-0001-6103-849X (Klapperich, Catherine); 766811 |
| DOI: | 10.1128/spectrum.01307-22 |
| الاتاحة: | https://hdl.handle.net/2144/45833 https://www.ncbi.nlm.nih.gov/pubmed/36255297 https://doi.org/10.1128/spectrum.01307-22 |
| Rights: | © 2022 Landaverde et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.F44EE3F |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 21650497 |
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| DOI: | 10.1128/spectrum.01307-22 |