Academic Journal
Identification and confirmation via in situ hybridization of Merkel cell polyomavirus in rare cases of posttransplant cutaneous T‐cell lymphoma
| العنوان: | Identification and confirmation via in situ hybridization of Merkel cell polyomavirus in rare cases of posttransplant cutaneous T‐cell lymphoma |
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| المؤلفون: | Lawrence, Lauren, Wang, Aihui, Charville, Gregory, Liu, Chih Long, Garofalo, Andrea, Alizadeh, Ash, Jangam, Diwash, Pinsky, Benjamin A., Sahoo, Malaya, Gratzinger, Dita, Khodadoust, Michael, Kim, Youn, Novoa, Roberto, Stehr, Henning |
| المصدر: | Journal of Cutaneous Pathology ; volume 50, issue 9, page 835-844 ; ISSN 0303-6987 1600-0560 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2023 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Background Viral infection is an oncogenic factor in many hematolymphoid malignancies. We sought to determine the diagnostic yield of aligning off‐target reads incidentally obtained during targeted hematolymphoid next‐generation sequencing to a large database of viral genomes to screen for viral sequences within tumor specimens. Methods Alignment of off‐target reads to viral genomes was performed using magicBLAST. Localization of Merkel cell polyomavirus (MCPyV) RNA was confirmed by RNAScope in situ hybridization. Integration analysis was performed using Virus‐Clip. Results Four cases of post‐cardiac‐transplant folliculotropic mycosis fungoides (fMF) and one case of peripheral T‐cell lymphoma (PTCL) were positive in off‐target reads for MCPyV DNA. Two of the four cases of posttransplant fMF and the case of PTCL showed localization of MCPyV RNA to malignant lymphocytes, whereas the remaining two cases of posttransplant fMF showed MCPyV RNA in keratinocytes. Conclusions Our findings raise the question of whether MCPyV may play a role in rare cases of T‐lymphoproliferative disorders, particularly in the skin and in the heavily immunosuppressed posttransplant setting. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/cup.14486 |
| الاتاحة: | http://dx.doi.org/10.1111/cup.14486 https://onlinelibrary.wiley.com/doi/pdf/10.1111/cup.14486 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.F4495278 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/cup.14486 |
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