التفاصيل البيبلوغرافية
| العنوان: |
Artificial Nanocage Formed via Self-Assembly of β‑Annulus Peptide for Delivering Biofunctional Proteins into Cell Interiors |
| المؤلفون: |
Kentarou Sakamoto (8828564), Hiroto Furukawa (11978201), Jan Vincent V. Arafiles (11879980), Miki Imanishi (210333), Kazunori Matsuura (2477176), Shiroh Futaki (210346) |
| سنة النشر: |
2022 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Microbiology, Cell Biology, Molecular Biology, Biotechnology, Immunology, Cancer, Inorganic Chemistry, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, nitrilotriacetic acid moiety, abovementioned requirements using, recipient cell cytosol, attain cell permeability, delivering biofunctional proteins, deliver functional proteins, amino acid peptide, successful intracellular delivery, intact proteins without, cell interiors nanocarriers, cargo protein encapsulation, intracellular delivery, cargo proteins, cell interiors, without compromising, tagged proteins, effective encapsulation, cytosolic proteins, annulus peptide |
| الوصف: |
Nanocarriers that deliver functional proteins to cell interiors are an attractive platform for the intracellular delivery of intact proteins without further modification, with in vivo compatibility. Development of efficient methods for cargo protein encapsulation and release in recipient cell cytosol is needed. Herein, we assess the feasibility of the abovementioned requirements using a protein nanocage (artificial nanocage) without compromising the structure and functions of the original protein and allowing for design flexibility of the surfaces and interiors. The protein nanocage formed via the self-assembly of the β-annulus peptide (24-amino acid peptide) in water was used as a model framework. The nitrilotriacetic acid moiety was displayed on the nanocage lumen for effective encapsulation of hexahistidine-tagged proteins in the presence of Ni 2+ , and the amphiphilic cationic lytic peptide HAad was displayed on a nanocage surface to attain cell permeability. Successful intracellular delivery of cargo proteins and targeting of cytosolic proteins by a nanobody were achieved, indicating the validity of the approach employed in this study. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Artificial_Nanocage_Formed_via_Self-Assembly_of_Annulus_Peptide_for_Delivering_Biofunctional_Proteins_into_Cell_Interiors/18784988 |
| DOI: |
10.1021/acs.bioconjchem.1c00534.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.bioconjchem.1c00534.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.F43B19BA |
| قاعدة البيانات: |
BASE |