التفاصيل البيبلوغرافية
| العنوان: |
Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench / |
| المؤلفون: |
Balčiūnaitė, Gabrielė, Juodsnukytė, Jovita, Savickas, Arūnas, Ragažinskienė, Ona, Šiatkutė, Luka, Žvirblytė, Gitana, Mištinienė, Edita, Savickienė, Nijolė |
| المصدر: |
Acta Pharmaceutica., Zagreb : Hrvatsko Farmaceutsko Drustvo, 2015, Vol. 65, Iss. 4, p. 473-479. ; ISSN 1330-0075 ; eISSN 1846-9558 |
| سنة النشر: |
2015 |
| المجموعة: |
Vilnius University Virtual Library (VU VL) / Vilniaus universitetas virtuali biblioteka |
| مصطلحات موضوعية: |
Echinacea purpurea (L.) Moench, proteins, gel filtration chromatography, SDS-PAGE |
| الوصف: |
Echinacea purpurea (L.) Moench, a member of the Asteraceae family, is a plant rich in flavonoids, essential oils, phenolic compounds, saponins, polysaccharides and glycoproteins. The aim of the study was to evaluate the protein content in dried roots of Echinacea purpurea (L.) Moench after homogenization of roots with liquid nitrogen, extraction in 0.01 mol L–1 phosphate-buffered saline (PBS) and purification followed by fractionation of proteins using gel filtration chromatography. Total concentration of proteins was measured using the Bradford method, and evaluation of the molecular mass of proteins was accomplished by applying the SDS-PAGE gel electrophoresis. The Bradford assay revealed that the highest concentration of proteins in fractions collected after gel filtration chomatography was 4.66–6.07 mg mL–1. Glycoproteins, alkamides and polysaccharides in roots of Echinacea purpurea (L.) Moench are chemical compounds that are responsible for their immunomodulatory properties. However, information about the difference of protein contents in fresh and dried roots of E. purpurea is insufficient. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
https://epublications.vu.lt/object/elaba:15316023/15316023.pdf; https://repository.vu.lt/VU:ELABAPDB15316023&prefLang=en_US |
| الاتاحة: |
https://repository.vu.lt/VU:ELABAPDB15316023&prefLang=en_US |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.F3F7F591 |
| قاعدة البيانات: |
BASE |