Academic Journal
The scaffolding protein flot2 promotes cytoneme-based transport of wnt3 in gastric cancer
| العنوان: | The scaffolding protein flot2 promotes cytoneme-based transport of wnt3 in gastric cancer |
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| المؤلفون: | Routledge, D, Rogers, S, Ono, Y, Brunt, L, Meniel, V, Tornillo, G, Ashktorab, H, Phesse, TJ, Scholpp, S |
| بيانات النشر: | eLife Sciences Publications |
| سنة النشر: | 2022 |
| المجموعة: | University of Exeter: Open Research Exeter (ORE) |
| مصطلحات موضوعية: | Wnt, cancer biology, cell biology, cytoneme, gastric cancer, lipid rafts, plasma membrane, signalling, zebrafish, Animals, Embryonic Development, Stomach Neoplasms, Wnt Proteins, Wnt Signaling Pathway |
| الوصف: | This is the final version. Available on open access from eLife Sciences Publications via the DOI in this record ; Data availability: All data generated or analysed during this study are included in the manuscript, supporting files and source files; Supporting Data files and Source Data have been provided to all figures. ; The Wnt/β-catenin signalling pathway regulates multiple cellular processes during development and many diseases, including cell proliferation, migration, and differentiation. Despite their hydrophobic nature, Wnt proteins exert their function over long distances to induce paracrine signalling. Recent studies have identified several factors involved in Wnt secretion; however, our understanding of how Wnt ligands are transported between cells to interact with their cognate receptors is still debated. Here, we demonstrate that gastric cancer cells utilise cytonemes to transport Wnt3 intercellularly to promote proliferation and cell survival. Furthermore, we identify the membrane-bound scaffolding protein Flotillin-2 (Flot2), frequently overexpressed in gastric cancer, as a modulator of these cytonemes. Together with the Wnt co-receptor and cytoneme initiator Ror2, Flot2 determines the number and length of Wnt3 cytonemes in gastric cancer. Finally, we show that Flotillins are also necessary for Wnt8a cytonemes during zebrafish embryogenesis, suggesting a conserved mechanism for Flotillin-mediated Wnt transport on cytonemes in development and disease. ; Medical Research Council (MRC) ; Biotechnology and Biological Sciences Research Council (BBSRC) |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | e77376-; Electronic |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://www.ncbi.nlm.nih.gov/pubmed/36040316; Elife, 11; orcid:0000-0002-4901-4740 (Rogers, Sally); orcid:0000-0002-7257-0120 (Ono, Yosuke); ScopusID: 12041146800 (Ono, Yosuke); orcid:0000-0002-4903-9657 (Scholpp, Steffen); ScopusID: 6508312503 (Scholpp, Steffen); ResearcherID: E-4472-2018 %7C H-2060-2013 (Scholpp, Steffen); Vol. 11, article e77376; https://doi.org/10.7554/eLife.77376; MR/N0137941/1; MR/S007970/1; MR/R026424/1; BB/S016295/1; http://hdl.handle.net/10871/131243; eLife |
| DOI: | 10.7554/eLife.77376 |
| الاتاحة: | http://hdl.handle.net/10871/131243 https://doi.org/10.7554/eLife.77376 |
| Rights: | © 2022, Routledge et al. Open access. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. ; https://creativecommons.org/licenses/by/4.0/ ; CC BY |
| رقم الانضمام: | edsbas.F3E25862 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.77376 |