Academic Journal
Peroxisome proliferator-activated receptor-γ activation induces 11β-hydroxysteroid dehydrogenase type 1 activity in human alternative macrophages.
| العنوان: | Peroxisome proliferator-activated receptor-γ activation induces 11β-hydroxysteroid dehydrogenase type 1 activity in human alternative macrophages. |
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| المؤلفون: | Chinetti-Gbaguidi, Giulia, Bouhlel, Mohamed Amine, Copin, Corinne, Duhem, Christian, Derudas, Bruno, Neve, Bernardette, Noel, Benoit, Eeckhoute, Jerome, Lefebvre, Philippe, Seckl, Jonathan, R., Staels, Bart |
| المساهمون: | Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 (RNMCD), Institut Pasteur de Lille, Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille), Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 (EGENODIA (GI3M)), Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Endocrinology Unit, University of Edinburgh (Edin.)-Queen's Medical Researche Institute, University of Edinburgh (Edin.), This work was supported by grants from the Nouvelle Société Française d'Athérosclérose/Sanofi-Aventis (to M.A.Bouhlel), the Région Nord-Pas de Calais/FEDER (ChoMetAlt project), the Fondation Coeur et Artères and theAgence Nationale de la Recherche (AlMHA project). G. Chinetti-Gbaguidi is a recipient of aContrat d'Interface from the CHRU de Lille. |
| المصدر: | ISSN: 1079-5642. |
| بيانات النشر: | CCSD American Heart Association |
| سنة النشر: | 2012 |
| المجموعة: | LillOA (HAL Lille Open Archive, Université de Lille) |
| مصطلحات موضوعية: | human alternative macrophages, inflammation, GR, 11beta-HSDI, PPARgamma, MESH: 11-beta-Hydroxysteroid Dehydrogenase Type 1, MESH: Cells, Cultured, MESH: PPAR gamma, MESH: RNA Interference, MESH: Receptors, Glucocorticoid, MESH: Thiazolidinediones, MESH: Time Factors, MESH: Transfection, MESH: Cortisone, MESH: Enzyme Induction, MESH: Genes, Reporter, MESH: Humans, MESH: Hydrocortisone, MESH: Inflammation, MESH: Interleukin-4, MESH: Macrophages, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology |
| الوصف: | International audience ; OBJECTIVE: 11β-Hydroxysteroid dehydrogenase type 1 (11β-HSD1) catalyzes the intracellular reduction of inactive cortisone to active cortisol, the natural ligand activating the glucocorticoid receptor (GR). Peroxisome proliferator- activated receptor-γ (PPARγ) is a nuclear receptor controlling inflammation, lipid metabolism, and the macrophage polarization state. In this study, we investigated the impact of macrophage polarization on the expression and activity of 11β-HSD1 and the role of PPARγ therein. METHODS AND RESULTS: 11β-HSD1 gene expression is higher in proinflammatory M1 and anti-inflammatory M2 macrophages than in resting macrophages, whereas its activity is highest in M2 macrophages. Interestingly, PPARγ activation induces 11β-HSD1 enzyme activity in M2 macrophages but not in resting macrophages or M1 macrophages. Consequently, human M2 macrophages displayed enhanced responsiveness to the 11β-HSD1 substrate cortisone, an effect amplified by PPARγ induction of 11β-HSD1 activity, as illustrated by an increased expression of GR target genes. CONCLUSION: Our data identify a positive cross-talk between PPARγ and GR in human M2 macrophages via the induction of 11β-HSD1 expression and activity. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/22207732; PUBMED: 22207732 |
| DOI: | 10.1161/ATVBAHA.111.241364 |
| الاتاحة: | https://inserm.hal.science/inserm-00656065 https://inserm.hal.science/inserm-00656065v1/document https://inserm.hal.science/inserm-00656065v1/file/inserm-00656065_edited.pdf https://inserm.hal.science/inserm-00656065v1/file/Bouhlel_dec2011.pdf https://inserm.hal.science/inserm-00656065v1/file/supplement_tables_legends_and_figures_R1.pdf https://doi.org/10.1161/ATVBAHA.111.241364 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.F36E8DBF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1161/ATVBAHA.111.241364 |
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