Academic Journal
Renal development / Cystic diseases
| العنوان: | Renal development / Cystic diseases |
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| المؤلفون: | Yosypiv, Ihor, Song, Renfang, Preston, Graeme, Van Eerde, Albertien M., Van Binsbergen, Ellen, Konijnenberg, Yvette, Maiburg, Merel C., Lichtenbelt, Klaske, Nikkels, Peter G.J., Vd Smagt, Jasper, Renkema, Kirsten Y., Giltay, Jacques C., De Jong, Tom P.V.M., Lilien, Marc R., Knoers, Nine V.A.M., Gueydan, Charlotte, Serena, Germano, Stephan, Grouls, Koesters, Robert, Zeineb, Bakey, Laure, Delestré, Catherine, Arnould, Marie-Thérése, Bihoreau, Gauguier, Dominique, Lelongt, Brigitte, Moon, Seung Hwan, Park, Hayne Cho, Lee, Ho-Young, Hwang, Jin Ho, Jeong, Jong Cheol, Park, Jae-Yoon, Lee, Seong Woo, Hwang, Young-Hwan, Kang, Keon Wook, Ahn, Curie, Gattone, Vincent, Carr, Alexander, Crosler-Roberts, Robin, Wang, Xueqi, Liu, Yang, Shen, Jianhua, Wuthrich, Rudolf, Serra, Andreas, Mei, Changlin, Tuta, Liliana, Botea, Florin, Guigonis, Vincent, Rodier, Nicolas, Bahans, Claire, Decramer, Stephane, Bertholet-Thomas, Aurelia, Heidet, Laurence, Eckart, Philippe, Lavocat, Marie-Pierre, Vrillon, Isabelle, Cloarec, Sylvie, Lahoche, Annie, Bessenay, Lucie, Louillet, Ferielle, Roussey, Gwenaelle, Rousset-Riviére, Caroline, Dunand, Olivier, Baudouin, Véronique, Nobili, François, Pietrement, Christine, De Parscau, Loïc, Gajdos, Vincent, Morin, Denis, Laffargue, Fanny, Llanas, Brigitte, Palcoux, Jean-Bernard, Delrue, Marie-Ange, Dizier, Emilie, Taupiac, Elisabeth, Laroche, Cecile, Lacombe, Bertrand, Bourthoumieu, Sylvie, El-Meanawy, Ashraf, Rufanova, Victoriya, Stelloh, Cary |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2012 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Abstracts |
| الوصف: | Introduction and Aims: Angiotensin (Ang)-(1-7) acts via the G protein-coupled receptor Mas to oppose Ang II/AT1 receptor-mediated effects on cell proliferation and cardiovascular homeostasis. In the present study, we examined the role of the Mas receptor in early UB branching morphogenesis and GDNF/Ret/Wnt11 pathway gene expression. Methods: Paired CD1 mice metanephroi were dissected on embryonic (E) day E12.5 and grown on polycarbonate transwell filters located on top of DMEM/F12 medium alone (control, n=5) or in the presence of the specific Mas receptor antagonist A-779 (10-6 M, n=5) for 24 hours. In addition, we examined UB branching in metanephroi from Mas +/+ (n=4) and Mas -/- (n=4) mice on E13.5. The UB epithelia were visualized by staining the metanephroi with anti- pancytokeratin antibody (1:200, Sigma) and the absolute number of UB tips in each group was counted. Results: A-779 increased the number of UB tips compared to control at 24 (41.2±2.7 vs. 31.2±1.9; p<0.05). Similar increase in the UB tip number was observed in Mas-/- compared with Mas+/+ kidneys (43.1±1.5 vs. 34.7±1.3; p<0.01). To determine whether the observed induction of UB branching by A-779 is due to upregulation of the GDNF/Ret/Wnt11 morphogenetic pathway, we measured GDNF, Ret and Wnt11 mRNA levels after 24 hours of culture by real-time quantitative PCR. Treatment with A-779 (n=3) significantly increased GDNF (1.88±0.2 vs. 1.0±0.0; p<0.05), Ret (1.62±0.1 vs. 1.0±0.0; p<0.01) and Wnt11 (1.91±0.1 vs. 1.0±0.0; p<0.01) mRNA levels in whole metanephroi compared to control (n=3). In contrast, A-779 decreased expression of Spry1 (0.32±0.02 vs. 1.0±0.0; p<0.01), an endogenous inhibitor of the GDNF/Ret/Wnt11 pathway. Conclusions: These findings indicate that endogenous Ang-(1-7) and its Mas receptor counteract the effects of Ang II/AT1 receptor axis on UB branching and GDNF/Ret/Wnt11 pathway gene expression. These results support the hypothesis that abnormal collecting system development in AT1 receptor-deficient mice may be at ... |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://ndt.oxfordjournals.org/cgi/content/short/27/suppl_2/ii317; http://dx.doi.org/10.1093/ndt/gfs231 |
| DOI: | 10.1093/ndt/gfs231 |
| الاتاحة: | http://ndt.oxfordjournals.org/cgi/content/short/27/suppl_2/ii317 https://doi.org/10.1093/ndt/gfs231 |
| Rights: | Copyright (C) 2012, European Renal Association - European Dialysis and Transplant Association |
| رقم الانضمام: | edsbas.F337DA89 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/ndt/gfs231 |
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