Academic Journal
High-field magnetic resonance microscopy of aortic plaques in a mouse model of atherosclerosis
| العنوان: | High-field magnetic resonance microscopy of aortic plaques in a mouse model of atherosclerosis |
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| المؤلفون: | Castro, Rita, Gullette, Sean, Whalen, Courtney, Mattie, Floyd J., Ge, Ximing, Ross, A. Catharine, Neuberger, Thomas |
| المصدر: | Magnetic Resonance Materials in Physics, Biology and Medicine ; volume 36, issue 6, page 887-896 ; ISSN 1352-8661 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2023 |
| الوصف: | Objectives Pre-clinical models of human atherosclerosis are extensively used; however, traditional histological methods do not allow for a holistic view of vascular lesions. We describe an ex-vivo, high-resolution MRI method that allows the 3 dimensional imaging of the vessel for aortic plaque visualization and quantification. Materials and methods Aortas from apolipoprotein-E-deficient ( apoE −/− ) mice fed an atherogenic diet (group 1) or a control diet (group 2) were subjected to 14 T MR imaging using a 3D gradient echo sequence. The obtained data sets were reconstructed (Matlab), segmented, and analyzed (Avizo). The aortas were further sectioned and subjected to traditional histological analysis (Oil-Red O and hematoxylin staining) for comparison. Results A resolution up to 15 × 10x10 μm 3 revealed that plaque burden (mm 3 ) was significantly ( p < 0.05) higher in group 1 (0.41 ± 0.25, n = 4) than in group 2 (0.01 ± 0.01, n = 3). The achieved resolution provided similar detail on the plaque and the vessel wall morphology compared with histology. Digital image segmentation of the aorta's lumen, plaque, and wall offered three-dimensional visualizations of the entire, intact aortas. Discussion 14 T MR microscopy provided histology-like details of pathologically relevant vascular lesions. This work may provide the path research needs to take to enable plaque characterization in clinical applications. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1007/s10334-023-01102-1 |
| DOI: | 10.1007/s10334-023-01102-1.pdf |
| DOI: | 10.1007/s10334-023-01102-1/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1007/s10334-023-01102-1 https://link.springer.com/content/pdf/10.1007/s10334-023-01102-1.pdf https://link.springer.com/article/10.1007/s10334-023-01102-1/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.F31CFE5A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s10334-023-01102-1 |
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