Academic Journal
Effect of Hedyotis diffusa water extract on protecting human hepatocyte cells (LO2) from H2O2-induced cytotoxicity
| العنوان: | Effect of Hedyotis diffusa water extract on protecting human hepatocyte cells (LO2) from H2O2-induced cytotoxicity |
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| المؤلفون: | Gao, Xin, Li, Chang, Tang, Yee Ling, Zhang, Huan, Chan, Shun Wan |
| المصدر: | Faculty of Science & Technology (THEi) |
| بيانات النشر: | VTC Institutional Repository |
| سنة النشر: | 2016 |
| المجموعة: | VTC Institutional Repository (Vocational Training Council) |
| مصطلحات موضوعية: | Apoptosis, Liver disease, Reactive oxygen species, Traditional Chinese medicinal herb, Pharmacy and Pharmaceutical Sciences |
| الوصف: | Context: Natural products are good sources of natural dietary antioxidants that are believed to protect the body against hepatotoxic effect induced by oxidative stress. Hedyotis diffusa Willd (Rubiaceae) (HDW) is a traditional Chinese medicinal herb that has been shown to possess a variety of antioxidant properties. Objective: The present study examines and explains the cell protective property of HDW water extract (WEHDW). Materials and Methods: 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) assay was used to measure the free radical scavenging property of WEHDW (0.001–10 mg/mL). The protective effect of WEHDW (0.3–10 mg/mL 2 h pretreatment) against hydrogen peroxide (H2O2, 200 μM for 6 h) induced cytotoxicity in human hepatic cells, LO2, was evaluated using cell viability assay and nuclear staining. The molecular pathway of WEHDW's effect was investigated by using Western blot assay. Results: WEHDW had a 50% scavenging concentration (SC50) at 0.153 mg/mL in the DPPH assay. Exposure of LO2 cells to H2O2 resulted in apoptosis which could be markedly attenuated by pre-treating WEHDW in a concentration-dependent manner (0.5, 1, 3, 5, or 10 mg/mL) (all with p < 0.001, versus control). Moreover, Hoechst (nuclear) staining showed that 1 mg/mL WEHDW could protect LO2 cells by attenuating apoptotic cell death mediated by H2O2. It was found that WEHDW reversed H2O2-induced activation of MEK/ERK pathway and H2O2-induced inhibition of P13-K/AKT/GSK3β pathway in LO2 cells. Discussion and Conclusion: WEHDW may help to improve the antioxidant defense system, resulting in prevention of oxidative stress-related fatty liver diseases. |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| Relation: | https://repository.vtc.edu.hk/thei-fac-sci-tech-sp/35 |
| DOI: | 10.3109/13880209.2015.1056310 |
| الاتاحة: | https://repository.vtc.edu.hk/thei-fac-sci-tech-sp/35 https://doi.org/10.3109/13880209.2015.1056310 |
| رقم الانضمام: | edsbas.F31284C9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3109/13880209.2015.1056310 |
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