Academic Journal
Phosphoproteomic analysis of the response to DNA damage in Trypanosoma brucei
| العنوان: | Phosphoproteomic analysis of the response to DNA damage in Trypanosoma brucei |
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| المؤلفون: | Mclaughlin, Emilia, Zavala Martinez, Monica Gabriela, Dujeancourt-Henry, Annick, Chaze, Thibault, Gianetto, Quentin Giai, Matondo, Mariette, Urbaniak, Michael, D, Glover, Lucy |
| المساهمون: | Biologie moléculaire des Trypanosomes - Trypanosome Molecular Biology, Institut Pasteur Paris (IP)-Université Paris Cité (UPCité), Collège Doctoral, Sorbonne Université (SU), Plateforme de Protéomique / Proteomics platform, Université Paris Cité (UPCité)-Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology (UTechS MSBio), Institut Pasteur Paris (IP)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut Pasteur Paris (IP)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Lancaster University, Work in the LG laboratory has received financial support from the Institut Pasteur. EJM is part of the Pasteur-Paris University (PPU) International PhD Program. This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 665807 and from the Foundation Recherché Médicale grant number FDT202012010602. M. G. Z. M. is funded by Agence Nationale de la Recherche (ANR, https://anr.fr/) through the ParaFrap "Laboratoire d’Excellence" (LabEx, https://www.enseignementsuprecherche.gouv.fr/cid51355/laboratoires-d-excellence.html) (ANR-11-LABX-0024). Funding for open access charge: Institut Pasteur core funding., ANR-11-LABX-0024,ParaFrap,Alliance française contre les maladies parasitaires(2011), European Project: 665807,H2020,H2020-MSCA-COFUND-2014,PASTEURDOC(2015) |
| المصدر: | ISSN: 0021-9258. |
| بيانات النشر: | CCSD American Society for Biochemistry and Molecular Biology |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | DNA break, DNA damage response, Trypanosoma brucei, phosphoproteomics, [SDV]Life Sciences [q-bio], [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] |
| الوصف: | International audience ; Damage to the genetic material of the cell poses a universal threat to all forms of life. The DNA damage response is a coordinated cellular response to a DNA break, key to which is the phosphorylation signaling cascade. Identifying which proteins are phosphorylated is therefore crucial to understanding the mechanisms that underlie it. We have used stable isotopic labeling of amino acids in cell culture-based quantitative phosphoproteomics to profile changes in phosphorylation site abundance following double stranded DNA breaks, at two distinct loci in the genome of the single cell eukaryote Trypanosoma brucei. Here, we report on the T. brucei phosphoproteome following a single double-strand break at either a chromosome internal or subtelomeric locus, specifically the bloodstream form expression site. We detected >6500 phosphorylation sites, of which 211 form a core set of double-strand break responsive phosphorylation sites. Along with phosphorylation of canonical DNA damage factors, we have identified two novel phosphorylation events on histone H2A and found that in response to a chromosome internal break, proteins are predominantly phosphorylated, while a greater proportion of proteins dephosphorylated following a DNA break at a subtelomeric bloodstream form expression site. Our data represent the first DNA damage phosphoproteome and provides novel insights into repair at distinct chromosomal contexts in T. brucei. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/39128729; info:eu-repo/grantAgreement//665807/EU/Institut Pasteur International Docotal Program/PASTEURDOC; PUBMED: 39128729; PUBMEDCENTRAL: PMC11408851 |
| DOI: | 10.1016/j.jbc.2024.107657 |
| الاتاحة: | https://pasteur.hal.science/pasteur-04854518 https://pasteur.hal.science/pasteur-04854518v1/document https://pasteur.hal.science/pasteur-04854518v1/file/PIIS0021925824021586.pdf https://doi.org/10.1016/j.jbc.2024.107657 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.F262481E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jbc.2024.107657 |
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