Academic Journal
Comparison of Processing and Sectioning Methodologies for Arteries Containing Metallic Stents
| العنوان: | Comparison of Processing and Sectioning Methodologies for Arteries Containing Metallic Stents |
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| المؤلفون: | Rippstein, Peter, Black, Melanie K., Boivin, Marie, Veinot, John P., Ma, Xiaoli, Chen, Yong-Xiang, Human, Paul, Zilla, Peter, O'Brien, Edward R. |
| المصدر: | Journal of Histochemistry & Cytochemistry ; volume 54, issue 6, page 673-681 ; ISSN 0022-1554 1551-5044 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2006 |
| الوصف: | The histological study of arteries with implanted metallic scaffolding devices, known as stents, remains a technical challenge. Given that the arterial response to stent implantation can sometimes lead to adverse outcomes, including the re-accumulation of tissue mass within the stent (or in-stent restenosis), overcoming these technical challenges is a priority for the advancement of research and development in this important clinical field. Essentially, the task is to section the stent-tissue interface with the least amount of disruption of tissue and cellular morphology. Although many methacrylate resin methodologies are successfully applied toward the study of endovascular stents by a variety of research laboratories, the exact formulations, as well as subsequent processing and sectioning methodology, remain largely coveted. In this paper, we describe in detail a methyl methacrylate resinembedding methodology that can successfully be applied to tungsten carbide blade, as well as saw and grinding sectioning methods and transmission electron microscopy. In addition, we present a comparison of the two sectioning methodologies in terms of their effectiveness with regard to morphological, histochemical, and immunohistochemical analyses. This manuscript contains online supplemental material at http://www.jhc.org . Please visit this article online to view these materials. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1369/jhc.5a6824.2006 |
| DOI: | 10.1369/jhc.5A6824.2006 |
| الاتاحة: | https://doi.org/10.1369/jhc.5a6824.2006 https://journals.sagepub.com/doi/pdf/10.1369/jhc.5A6824.2006 https://journals.sagepub.com/doi/full-xml/10.1369/jhc.5A6824.2006 |
| Rights: | https://journals.sagepub.com/page/policies/text-and-data-mining-license |
| رقم الانضمام: | edsbas.F238E29F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1369/jhc.5a6824.2006 |
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