التفاصيل البيبلوغرافية
| العنوان: |
NRX-1 localization to cholinergic axons is decreased in unc-104 mutants and colocalizes with UNC-104 in cholinergic axon terminals. |
| المؤلفون: |
Devyn Oliver (12015660), Shankar Ramachandran (7962029), Alison Philbrook (12015663), Christopher M. Lambert (12015666), Ken C. Q. Nguyen (12015669), David H. Hall (12015672), Michael Francis (287202) |
| سنة النشر: |
2022 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Cell Biology, Neuroscience, Developmental Biology, Inorganic Chemistry, Mental Health, stabilize circuit connectivity, gabaergic motor neurons, div >< p, partnering neurons, vivo <, temporal order, systemically examined, synaptic connections, synaptic activity, synapse formation, strict requirement, presynaptic terminals, postsynaptic structure, postsynaptic maintenance, poorly understood, neural circuits, mature animals, maintenance events, functional properties, elegans <, dendritic spines, collapse prior, c < |
| الوصف: |
(A) Quantification of NRX-1::GFP (endogenous knock-in) clusters in a 50 μm region of the dorsal nerve cord in wild type and unc-104(e1265) animals. Bars, mean ± SEM. Student’s t-test, ****p <0.0001. n ≥ 11 animals. (B) Quantification of NRX-1::GFP (endogenous knock-in) axon fluorescence intensity in a 50 μm region of the dorsal nerve cord. Bars, mean ± SEM. Student’s t-test, ****p <0.0001. n ≥ 11 animals. (C) Fluorescent images of NRX-1::GFP (endogenous knock-in) in somas of wild type and unc-104(e1265) mutants. Dotted white lines outline the neuronal cell body. (D) Quantification of NRX-1::GFP soma fluorescence intensity. Bars, mean ± SEM. Student’s test, ****p <0.001. (E) Quantification of the number of NRX-1::GFP (P unc129 ::NRX-1::GFP) clusters in a 50 μm region of the dorsal nerve cord in wild type, unc-104(e1265) , and unc-104(e1265) mutants rescued with cholinergic expression of wild type unc-104 . Bars, mean ± SEM. One-way ANOVA, Dunnett’s multiple comparisons test, ****p <0.0001. n ≥ 11 animals. (F) Quantification of NRX-1::GFP fluorescence intensity in cholinergic axons (P unc129 ::NRX-1::GFP) of a 50 μm region of the dorsal nerve cord. Bars, mean ± SEM. One-way ANOVA, Dunnett’s multiple comparisons test, ****p <0.0001. n ≥ 11 animals. (G) Fluorescent images of NRX-1::GFP in cholinergic somas of wild type, unc-104(e1265) and unc-104(e1265) mutants rescued with cholinergic expression of wild type unc-104 . Dotted white lines outline the neuronal cell body. (H) Quantification of NRX-1::GFP fluorescence intensity in cholinergic somas. Bars, mean ± SEM. One-way ANOVA, Dunnett’s multiple comparisons test, ****p <0.001. (I) Line scans depicting relative fluorescent intensity of NRX-1::GFP (green) and UNC-104::mCherry (red) for a 50 μm region of the dorsal nerve cord. Gray dotted rectangles indicate corresponding puncta in S11J Fig . (J) Fluorescent images of the dorsal nerve cord in an adult animal expressing NRX-1::GFP (P unc-129 ::NRX-1::GFP) and UNC-104::mCherry (P unc-129 ... |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/NRX-1_localization_to_cholinergic_axons_is_decreased_in_i_unc-104_i_mutants_and_colocalizes_with_UNC-104_in_cholinergic_axon_terminals_/19089513 |
| DOI: |
10.1371/journal.pgen.1010016.s012 |
| الاتاحة: |
https://doi.org/10.1371/journal.pgen.1010016.s012 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.F22961CE |
| قاعدة البيانات: |
BASE |