Academic Journal
Identification of drought-responsive genes in roots of upland rice ( Oryza sativa L)
| العنوان: | Identification of drought-responsive genes in roots of upland rice ( Oryza sativa L) |
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| المؤلفون: | Brasileiro Ana CM, de Souza Emanuel, Seixas Daniela, da Silva Felipe R, Rangel Paulo HN, Guimarães Cléber M, Rabello Aline R, Spehar Carlos R, Ferreira Márcio E, Mehta Ângela |
| المصدر: | BMC Genomics, Vol 9, Iss 1, p 485 (2008) |
| بيانات النشر: | BMC |
| سنة النشر: | 2008 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Biotechnology, TP248.13-248.65, Genetics, QH426-470 |
| الوصف: | Background Rice ( Oryza sativa L.) germplasm represents an extraordinary source of genes that control traits of agronomic importance such as drought tolerance. This diversity is the basis for the development of new cultivars better adapted to water restriction conditions, in particular for upland rice, which is grown under rainfall. The analyses of subtractive cDNA libraries and differential protein expression of drought tolerant and susceptible genotypes can contribute to the understanding of the genetic control of water use efficiency in rice. Results Two subtractive libraries were constructed using cDNA of drought susceptible and tolerant genotypes submitted to stress against cDNA of well-watered plants. In silico analysis revealed 463 reads, which were grouped into 282 clusters. Several genes expressed exclusively in the tolerant or susceptible genotypes were identified. Additionally, proteome analysis of roots from stressed plants was performed and 22 proteins putatively associated to drought tolerance were identified by mass spectrometry. Conclusion Several genes and proteins involved in drought-response, as well as genes with no described homologs were identified. Genes exclusively expressed in the tolerant genotype were, in general, related to maintenance of turgor and cell integrity. In contrast, in the susceptible genotype, expression of genes involved in protection against cell damage was not detected. Several protein families identified in the proteomic analysis were not detected in the cDNA analysis. There is an indication that the mechanisms of susceptibility to drought in upland rice are similar to those of lowland varieties. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1471-2164 |
| Relation: | http://www.biomedcentral.com/1471-2164/9/485; https://doaj.org/toc/1471-2164; https://doaj.org/article/d8482007f1ff4db8b5463c6673ab62a3 |
| DOI: | 10.1186/1471-2164-9-485 |
| الاتاحة: | https://doi.org/10.1186/1471-2164-9-485 https://doaj.org/article/d8482007f1ff4db8b5463c6673ab62a3 |
| رقم الانضمام: | edsbas.F1EB989E |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14712164 |
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| DOI: | 10.1186/1471-2164-9-485 |