Academic Journal
Microbial population heterogeneity versus bioreactor heterogeneity: evaluation of Redox Sensor Green as an exogenous metabolic biosensor
| العنوان: | Microbial population heterogeneity versus bioreactor heterogeneity: evaluation of Redox Sensor Green as an exogenous metabolic biosensor |
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| المؤلفون: | Baert, Jonathan, Delepierre, Anissa, Telek, Samuel, Fickers, Patrick, Toye, Dominique, Delamotte, Anne, Lara, Alvaro R., Jaén, Karim E., Gosset, Guillermo, Jensen, Peter Ruhdal, Delvigne, Frank |
| المصدر: | Baert , J , Delepierre , A , Telek , S , Fickers , P , Toye , D , Delamotte , A , Lara , A R , Jaén , K E , Gosset , G , Jensen , P R & Delvigne , F 2016 , ' Microbial population heterogeneity versus bioreactor heterogeneity: evaluation of Redox Sensor Green as an exogenous metabolic biosensor ' , Engineering in Life Sciences (Online) , vol. 16 , no. 7 , pp. 643-651 . https://doi.org/10.1002/elsc.201500149 |
| سنة النشر: | 2016 |
| المجموعة: | Technical University of Denmark: DTU Orbit / Danmarks Tekniske Universitet |
| مصطلحات موضوعية: | single cell, scale-down, biological noise, flow cytometry, phenotypic heterogeneity |
| الوصف: | Microbial heterogeneity in metabolic performances has attracted a lot of attention, considering its potential impact on industrial bioprocesses. However, little is known about the impact of extracellular perturbations (i.e. bioreactor heterogeneity) on cell-to-cell variability in metabolic performances (i.e. microbial population heterogeneity). In this work, we have evaluated the relevance of Redox Sensor Green (RSG) as an exogenous biosensor of metabolic activity at the single cell level. RSG signal is proportional to the activity of the electron transport chain and its signal is strongly affected by metabolic burden, availability of electron final acceptor and side metabolisms (i.e. overflow and mixed acid fermentation). RSG can also be used for the estimation of the impact of scale-down conditions on microbial metabolic robustness. The relationship linking averaged RSG activity and its cell-to-cell variability (noise) has been highlighted but seems unaffected by environmental perturbations. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://orbit.dtu.dk/en/publications/062a9e05-f97d-436f-9371-57a30d6e958c |
| DOI: | 10.1002/elsc.201500149 |
| الاتاحة: | https://orbit.dtu.dk/en/publications/062a9e05-f97d-436f-9371-57a30d6e958c https://doi.org/10.1002/elsc.201500149 https://backend.orbit.dtu.dk/ws/files/124296591/Baert_et_al_2016_Engineering_in_Life_Sciences.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.F1D3E02A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/elsc.201500149 |
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