Academic Journal
A New Nested Primer Pair Improves the Specificity of Ck-19 Mrna Detection by Rt-Pcr in Occult Breast Cancer Cells
| العنوان: | A New Nested Primer Pair Improves the Specificity of Ck-19 Mrna Detection by Rt-Pcr in Occult Breast Cancer Cells |
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| المؤلفون: | Balducci, E., Azzarello, G., Valori, L., Toffolatti, L., Bolgan, L., Valenti, M.T., Bari, M., Pappagallo, G.L., Ausoni, S., Vinante, O. |
| المصدر: | The International Journal of Biological Markers ; volume 20, issue 1, page 28-33 ; ISSN 0393-6155 1724-6008 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2005 |
| الوصف: | Reverse transcription polymerase chain reaction (RT-PCR) of cytokeratin-19 (CK-19) has been widely used to detect small numbers of circulating malignant epithelial cells in the bone marrow or the peripheral blood of patients with breast cancer. However, a high percentage of false positive results has been recorded and conflicting reports question the clinical relevance of this technical approach. We demonstrate that the use of a new nested primer pair for CK-19 RT-PCR avoids false positive results without affecting the sensitivity of the assay. Our experiments were carried out using MCF-7 cells alone or mixed with peripheral blood mononuclear cells (PBMNC) of healthy donors. The results were also validated in a large series of healthy donors and in a preliminary study on a limited number of patients with breast cancer, thus suggesting that our assay is feasible for application in the clinical evaluation of occult malignant epithelial cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/172460080502000105 |
| الاتاحة: | https://doi.org/10.1177/172460080502000105 https://journals.sagepub.com/doi/pdf/10.1177/172460080502000105 |
| Rights: | https://journals.sagepub.com/page/policies/text-and-data-mining-license |
| رقم الانضمام: | edsbas.F1CE8E41 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/172460080502000105 |
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