Academic Journal
Tryparedoxin peroxidases and superoxide dismutases expression as well as ROS release are related to Trypanosoma cruzi epimastigotes growth phases.
| العنوان: | Tryparedoxin peroxidases and superoxide dismutases expression as well as ROS release are related to Trypanosoma cruzi epimastigotes growth phases. |
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| المؤلفون: | Peloso, Eduardo F, Gonçalves, Conrado C, Silva, Thiago M, Ribeiro, Luis Henrique G, Piñeyro, María Dolores, Robello, Carlos, Gadelha, Fernanda R |
| المساهمون: | Departamento de Bioquimica, Universidade Estadual de Campinas = University of Campinas (UNICAMP), Institut Pasteur de Montevideo, Pasteur Network (Réseau International des Instituts Pasteur), Facultad de Medicina-Universidad de la República Montevideo (UDELAR), This work was supported by a Grant from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). E.F.P., C.C.G. and T.M.S. are students supported by Capes and FAPESP fellowships. |
| المصدر: | ISSN: 0003-9861. |
| بيانات النشر: | CCSD Elsevier |
| سنة النشر: | 2012 |
| المجموعة: | Réseau International des Instituts Pasteur, Paris: HAL-RIIP |
| مصطلحات موضوعية: | Trypanosoma cruzi, Reactive oxygen species, Growth curve, Antioxidant defenses, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology |
| الوصف: | International audience ; Trypanosoma cruzi's antioxidant system is unique and relevant to the parasite. In this study, quantitative assays were performed to determine cytosolic and mitochondrial tryparedoxin peroxidases and superoxide dismutases expression (TcCPx, TcMPx, SODB and SODA) in correlation to H(2)O(2) release and O(2)(-) production. Differences were observed regarding H(2)O(2) release and O(2)(-) production between strains and along the growth curve. All of the enzymes studied exhibited varied expression as a function of time in culture. Although at lower levels, the Y strain exhibited the same pattern of Tulahuen 2 enzyme expression for all of the proteins studied, except SODA. In the stationary phase, the degree of expression of all of the enzymes in the Y strain returned to similar levels as those detected in the log phase with the exception of TcCPx and SODA. In Tulahuen 2, a higher expression of TcMPx, SODA and SODB was detected in the early stationary phase, and a slight decrease was observed in the late stationary phase for each enzyme, excluding TcMPx, which exhibited a marked decrease, and TcCPx, which increased its level. Because of the significance of ROS in redox signaling, these differences in enzyme expression underscore the importance of these parameters for epimastigote proliferation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/22425758; PUBMED: 22425758 |
| DOI: | 10.1016/j.abb.2012.02.020 |
| الاتاحة: | https://riip.hal.science/pasteur-00685054 https://doi.org/10.1016/j.abb.2012.02.020 |
| رقم الانضمام: | edsbas.F1B5D2C5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.abb.2012.02.020 |
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