Academic Journal
Silver ion chromatography for peak resolution enhancement: Application to the preparative separation of two sesquiterpenes using online heart-cutting LC-LC technique
| العنوان: | Silver ion chromatography for peak resolution enhancement: Application to the preparative separation of two sesquiterpenes using online heart-cutting LC-LC technique |
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| المؤلفون: | Yang, Yang, Zhang, Yongmin, Wei, Chong, Li, Jing, Sun, Wenji |
| المساهمون: | Northwestern Polytechnical University Xi'an (NPU), Institut Parisien de Chimie Moléculaire (IPCM), Chimie Moléculaire de Paris Centre (FR 2769), École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris Sciences et Lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris Sciences et Lettres (PSL)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 0039-9140 ; Talanta ; https://hal.sorbonne-universite.fr/hal-01905869 ; Talanta, 2018, 187, pp.252-258. ⟨10.1016/j.talanta.2018.05.043⟩. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Peak resolution enhancement, Argentation, Positional isomers, Preparative separation, Natural product, Heart-cutting LC-LC, [CHIM.ANAL]Chemical Sciences/Analytical chemistry |
| الوصف: | International audience ; Silver ion chromatography, utilizing columns packed with silver ions bonded to silica gel, has proved to be an invaluable technique for the analysis of some positional isomers. In this work, silver ion chromatography by combination with online heart-cutting LC-LC technique for the preparative separation of two sesquiterpenes positional isomers from a natural product was investigated. On the basis of the evaluation that silver ion content impacts on the separation, the laboratory-made silver ion columns, utilizing silica gel impregnated with 15% silver nitrate as column packing materials, were used for peak resolution improvement of these two isomers and the preparative separation of them in heart-cutting LC-LC. The relationship among the maximal sample load, flow rate and peak resolution in the silver ion column were optimized, and the performance of the silver ion column was compared with conventional C18 column and silica gel column. Based on the developed chromatographic conditions, online heart-cutting LC-LC chromatographic separation system in combination with a silica gel column and a silver ion column that was applied to preparative separation of these two isomers from a traditional Chinese medicine, Inula racemosa Hook.f., was established. The results showed that the online heart-cutting LC-LC technique by combination of a silica gel column and a silver ion column for the preparative separation of these two positional isomers from this natural plant was superior to the preparative separation performed on a single-column system with C18 column or silica gel column. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-01905869; https://hal.sorbonne-universite.fr/hal-01905869; https://hal.sorbonne-universite.fr/hal-01905869/document; https://hal.sorbonne-universite.fr/hal-01905869/file/ZHANG_Yongmin.pdf |
| DOI: | 10.1016/j.talanta.2018.05.043 |
| الاتاحة: | https://hal.sorbonne-universite.fr/hal-01905869 https://hal.sorbonne-universite.fr/hal-01905869/document https://hal.sorbonne-universite.fr/hal-01905869/file/ZHANG_Yongmin.pdf https://doi.org/10.1016/j.talanta.2018.05.043 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.F1A0E7E6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.talanta.2018.05.043 |
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