Academic Journal
Dynamic changes of the extracellular matrix during corneal wound healing
| العنوان: | Dynamic changes of the extracellular matrix during corneal wound healing |
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| المؤلفون: | Lorenzo Martín, Elvira, Gallego Muñoz, Patricia, Mar Sardaña, Santiago, Fernández Martínez, Itziar, Cidad Velasco, María del Pilar, Martínez García, María del Carmen |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2019 |
| المجموعة: | UVaDOC - Repositorio Documental de la Universidad de Valladolid |
| مصطلحات موضوعية: | Extracellular matrix, Matriz extracelular, Collagen type III, Colágeno tipo III, Decorin, Decorina, Corneal wound healing, Cicatrización de herida corneal |
| الوصف: | Producción Científica ; The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans. We monitored the corneal wound healing process after alkali burns in rabbits. We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency. After the animals received both general and ocular topical anesthesia, the central cornea of the left eye was burned by placing an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s. The eyes were evaluated under a surgical microscope at 1, 3, and 6 months after burning. At each time point, the clinical conditions of the burned and control corneas were observed. The arrangement of collagen fibers in the corneal stroma was visualized by Picrosirius-red staining, Gomori's silver impregnation and transmission electronic microscopy. Corneal light transmittance was also measured. Myofibroblasts presence was analyzed by immunohistochemistry. mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction. One month after alkali burn, the ECM was disorganized and filled with lacunae containing different types of cells and collagen type III fibers in the wound area. Corneal opacities were present with attendant loss of light transmittance. Collagen and proteoglycan mRNA expression levels were up-regulated. After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels. After six months, the wound area ECM morphology was similar to controls, but transmittance values remained low, denoting incomplete restoration of the stromal architecture. This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0014-4835 |
| Relation: | https://www.sciencedirect.com/science/article/pii/S0014483519302040; https://doi.org/10.1016/j.exer.2019.107704; Experimental Eye Research, 2019, vol. 186; http://uvadoc.uva.es/handle/10324/37291 |
| DOI: | 10.1016/j.exer.2019.107704 |
| الاتاحة: | http://uvadoc.uva.es/handle/10324/37291 https://doi.org/10.1016/j.exer.2019.107704 |
| Rights: | Attribution-NonCommercial-NoDerivatives 4.0 Internacional ; info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by-nc-nd/4.0/ ; © 2019 Elsevier |
| رقم الانضمام: | edsbas.F1859396 |
| قاعدة البيانات: | BASE |
| تدمد: | 00144835 |
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| DOI: | 10.1016/j.exer.2019.107704 |