التفاصيل البيبلوغرافية
| العنوان: |
CXCL11/12-AF647 scavenging is abrogated in dermal LECs isolated from ACKR3 i Δ LEC animals. |
| المؤلفون: |
Elena C. Sigmund (5128667), Lilian Baur (10654171), Philipp Schineis (6582434), Jorge Arasa (6489023), Victor Collado-Diaz (8774711), Martina Vranova (6582440), Rolf A. K. Stahl (7359425), Marcus Thelen (164322), Cornelia Halin (574950) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Medicine, Cell Biology, Genetics, Molecular Biology, Physiology, Immunology, Developmental Biology, Cancer, Mental Health, Infectious Diseases, postnatal lymphatic development, CXCR, murine skin-derived LECs, ACKR 3, postnatal lymphangiogenesis, lymphatic drainage function, AM, mice Atypical chemokine receptor ACKR 3, LEC-expressed ACKR 3, murine lymphatic development, inducible ACKR 3 knockout mouse, peptide hormone adrenomedullin |
| الوصف: |
Dermal stromal cells were isolated from the ear skin of ACKR3 WT or ACKR3 i Δ LEC mice and their ability to scavenge the fluorescent ACKR3-specific chimeric chemokine CXCL11/12 was investigated in vitro . (A) . CXCL11/12-AF647 scavenging was specifically abrogated in dermal CD31 + Podoplanin + LECs isolated from ACKR3 i Δ LEC animals, or in LECs and CD31 + Podoplanin - BECs from ACKR3 WT mice upon treatment with the ACKR3-selective competitive agonist CCX771. Representative histograms from 1 out of 5 experiments are shown. The corresponding gating strategy is shown in S4 Fig A. 4°C CXCL11/12: Chemokine uptake was performed at 4°C (impaired uptake control). CXCL11/12 + CCX771: Chemokine uptake was performed in presence of CCX771. (B) Comparison of the mean fluorescence intensity (MFI) values measured in chemokine uptake assays performed in presence (+CCX771) or absence (CTRL) of CCX771 in LECs isolated from either ACKR3 WT or ACKR3 i Δ LEC mice, Wilcoxon matched-pairs test, n = 6 experiments. (C) Summary of the difference in MFI (ΔMFI) measured in the chemokine uptake assays performed in (B) in presence/absence of CCX771 in LECs isolated from either ACKR3 WT or ACKR3 i Δ LEC mice. Mann-Whitney U-test, n = 6 experiments are shown in (B) and (C). Each dot represents the value obtained in one experiment, involving cells isolated from one animal. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/CXCL11_12-AF647_scavenging_is_abrogated_in_dermal_LECs_isolated_from_i_ACKR3_i_sup_i_i_i_i_LEC_i_sup_animals_/14424967 |
| DOI: |
10.1371/journal.pone.0249068.g004 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0249068.g004 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.F18329DE |
| قاعدة البيانات: |
BASE |