Academic Journal
Detection of Banned Ruminant-Derived Material in Industrial Feedstuffs by TaqMan Real-Time PCR Assay
| العنوان: | Detection of Banned Ruminant-Derived Material in Industrial Feedstuffs by TaqMan Real-Time PCR Assay |
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| المؤلفون: | PEGELS, NICOLETTE, GONZÁLEZ, ISABEL, GARCÍA, TERESA, MARTÍN, ROSARIO |
| المصدر: | Journal of Food Protection ; volume 74, issue 8, page 1300-1308 ; ISSN 0362-028X 1944-9097 |
| بيانات النشر: | Elsevier BV |
| سنة النشر: | 2011 |
| المجموعة: | ScienceDirect (Elsevier - Open Access Articles via Crossref) |
| الوصف: | A ruminant-specific real-time PCR system was designed and applied for the detection of processed animal protein from ruminants in industrial feedstuffs. The assay includes a primer pair and a TaqMan probe selectively targeting mitochondrial 16S rRNA gene sequences from the ruminant group and another primer-probe set based on the eukaryotic nuclear 18S rRNA gene (positive amplification control). Both ruminant and eukaryotic PCR systems generated short PCR amplicons of 79 and 77 bp, respectively. To evaluate the suitability of the real-time PCR assay for the detection of banned by-products of ruminant origin, 126 feed samples subjected to rendering under current European legislation regulations were analyzed. The assay achieved 100% success in classifying the samples as positive or negative in terms of qualitative ruminant composition, with a detection limit of 0.1%. The quantitative ability of the assay is however restricted by variations in the composition and treatment of the feeds, which affect the amount and quality of amplifiable DNA. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.4315/0362-028x.jfp-11-029 |
| الاتاحة: | http://dx.doi.org/10.4315/0362-028x.jfp-11-029 http://meridian.allenpress.com/jfp/article-pdf/74/8/1300/1686441/0362-028x_jfp-11-029.pdf |
| رقم الانضمام: | edsbas.F18046E1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.4315/0362-028x.jfp-11-029 |
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