Academic Journal
Human apolipoprotein(a) kringle V inhibits angiogenesis in vitro and in vivo by interfering with the activation of focal adhesion kinases
| العنوان: | Human apolipoprotein(a) kringle V inhibits angiogenesis in vitro and in vivo by interfering with the activation of focal adhesion kinases |
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| المساهمون: | Jang-Seong Kim, Hyun-Kyung Yu, Jin-Hyung Ahn, Ho-Jeong Lee, Soon-Won Hong, Kyung-Hwan Jung, Soo-Ik Chang, Yong-Kil Hong, Young-Ae Joe, Si-Myung Byun, Suk-Keun Lee, Soo-Il Chung, Yeup Yoon, Hong, Soon Won |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Actins / metabolism, Amino Acid Sequence, Animals, Apolipoproteins / chemistry, Apoprotein(a), Blotting, Western, Cell Movement, Cells, Cultured, Chick Embryo, Chorion / metabolism, Collagen / pharmacology, DNA, Complementary / metabolism, Dose-Response Relationship, Drug, Drug Combinations, Electrophoresis, Polyacrylamide Gel, Endothelium, Vascular / cytology, Vascular / pathology, Focal Adhesion Kinase 1, Focal Adhesion Protein-Tyrosine Kinases, Genetic Vectors, Humans, Kringles, Laminin / pharmacology, Lipoprotein(a) / chemistry |
| الوصف: | Apolipoprotein(a) [apo(a)] contains the largest numbers of kringle domains identified to date. Of these, apo(a) kringle V shows significant sequence homology with plasminogen kringle 5, which is reported to be a potent angiogenesis inhibitor. To determine the effects of apo(a) kringle V on angiogenesis, it was expressed as a soluble protein (termed rhLK8) in Pichia pastoris and its in vitro and in vivo anti-angiogenic properties were examined. rhLK8 inhibited the migration of human umbilical vein endothelial cells in vitro in a dose-dependent manner. This function was associated with the down-regulation of the activation of focal adhesion kinase and the inhibition of the consequent formation of actin stress fibers/focal adhesions. rhLK8 also inhibited new capillary formation in vivo, as assessed by the chick chorioallantoic membrane assay and the Matrigel plug assay. These results indicate that rhLK8 may be an effective angiogenesis inhibitor both in vitro and in vivo. ; restriction |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0006-291X 1090-2104 |
| Relation: | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS; J00281; OAK-2020-02486; https://ir.ymlib.yonsei.ac.kr/handle/22282913/178805; https://www.sciencedirect.com/science/article/pii/S0006291X03025488; T200404950; BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.313(3) : 534-540, 2004-01 |
| DOI: | 10.1016/j.bbrc.2003.11.148 |
| الاتاحة: | https://ir.ymlib.yonsei.ac.kr/handle/22282913/178805 https://doi.org/10.1016/j.bbrc.2003.11.148 https://www.sciencedirect.com/science/article/pii/S0006291X03025488 |
| Rights: | CC BY-NC-ND 2.0 KR |
| رقم الانضمام: | edsbas.F1787CE0 |
| قاعدة البيانات: | BASE |
| تدمد: | 0006291X 10902104 |
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| DOI: | 10.1016/j.bbrc.2003.11.148 |