Academic Journal
Integration of stool microbiota, proteome and amino acid profiles to discriminate patients with adenomas and colorectal cancer
| العنوان: | Integration of stool microbiota, proteome and amino acid profiles to discriminate patients with adenomas and colorectal cancer |
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| المؤلفون: | Sofie Bosch, Animesh Acharjee, Mohammed Nabil Quraishi, Irene V Bijnsdorp, Patricia Rojas, Abdellatif Bakkali, Erwin EW Jansen, Pieter Stokkers, Johan Kuijvenhoven, Thang V Pham, Andrew D Beggs, Connie R Jimenez, Eduard A Struys, Georgios V Gkoutos, Tim GJ de Meij, Nanne KH de Boer |
| المصدر: | Gut Microbes, Vol 14, Iss 1 (2022) |
| بيانات النشر: | Taylor & Francis Group |
| سنة النشر: | 2022 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Colon cancer, adenoma, multi omics, data integration, biomarker, stool, Diseases of the digestive system. Gastroenterology, RC799-869 |
| الوصف: | Background Screening for colorectal cancer (CRC) reduces its mortality but has limited sensitivity and specificity. Aims We aimed to explore potential biomarker panels for CRC and adenoma detection and to gain insight into the interaction between gut microbiota and human metabolism in the presence of these lesions.Methods This multicenter case-control cohort was performed between February 2016 and November 2019. Consecutive patients ≥18 years with a scheduled colonoscopy were asked to participate and divided into three age, gender, body-mass index and smoking status-matched subgroups: CRC (n = 12), adenomas (n = 21) and controls (n = 20). Participants collected fecal samples prior to bowel preparation on which proteome (LC-MS/MS), microbiota (16S rRNA profiling) and amino acid (HPLC) composition were assessed. Best predictive markers were combined to create diagnostic biomarker panels. Pearson correlation-based analysis on selected markers was performed to create networks of all platforms.Results Combining omics platforms provided new panels which outperformed hemoglobin in this cohort, currently used for screening (AUC 0.98, 0.95 and 0.87 for CRC vs controls, adenoma vs controls and CRC vs adenoma, respectively). Integration of data sets revealed markers associated with increased blood excretion, stress- and inflammatory responses and pointed toward downregulation of epithelial integrity.Conclusions Integrating fecal microbiota, proteome and amino acids platforms provides for new biomarker panels that may improve noninvasive screening for adenomas and CRC, and may subsequently lead to lower incidence and mortality of colon cancer. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1949-0984 1949-0976 |
| Relation: | https://doaj.org/toc/1949-0976; https://doaj.org/toc/1949-0984; https://doaj.org/article/7b47430b742b473c9f1e78bcdb4e406c |
| DOI: | 10.1080/19490976.2022.2139979 |
| الاتاحة: | https://doi.org/10.1080/19490976.2022.2139979 https://doaj.org/article/7b47430b742b473c9f1e78bcdb4e406c |
| رقم الانضمام: | edsbas.F1335694 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19490984 19490976 |
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| DOI: | 10.1080/19490976.2022.2139979 |