Academic Journal
Effects of histone deacetylase inhibitor trichostatin A combined with cisplatin on apoptosis of A549 cell line
| العنوان: | Effects of histone deacetylase inhibitor trichostatin A combined with cisplatin on apoptosis of A549 cell line |
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| المؤلفون: | Zhang, Xuan, Jiang, Shu‐Juan, Shang, Bin, Jiang, Hong‐Juan |
| المصدر: | Thoracic Cancer ; volume 6, issue 2, page 202-208 ; ISSN 1759-7706 1759-7714 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2015 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Background Histone deacetylase ( HDAC ) inhibitors combined with other drugs for the treatment of malignant tumors are used more and more widely. In this study, we investigated the effect of trichostatin A ( TSA ), a HDAC inhibitor, in combination with cisplatin, a cytotoxic chemotherapy agent, on the apoptosis of lung cancer A 549 cells. Methods A 549 cells were treated with TSA alone, cisplatin alone or the two drugs combined. Cell viability and apoptosis were evaluated using a light microscope, methyl thiazolyl tetrazolium ( MTT) (3‐[4, 5‐dimethylthiazol‐2‐yl] −2, 5‐diphenyltetrazolium bromide) assay and H ochst33258 staining. Moreover, W estern blot analysis was employed to examine the alterations of apoptosis protein: cellular F as‐associated death domain‐like interleukin‐1 β converting enzyme inhibitory protein ( cFLIP ) and caspase‐8 in A 549 cells in response to the different exogenous stimuli. Results Compared with single‐agent treatment, the co‐treatment of A 549 cells with TSA and cisplatin synergistically inhibited cell proliferation, induced apoptosis, and increased the inhibition rate. Treatment with TSA and cisplatin led to a significant decrease of cFLIP expression. Furthermore, the treatment of A 549 cells with TSA and cisplatin resulted in a significant decrease of pro‐caspase‐8 and a significant increase of caspase‐8. Conclusions Synergistic anti‐tumor effects are observed between cisplatin and TSA in lung cancer cells. The combination of TSA with cisplatin may be a more effective method in human lung cancer treatment. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/1759-7714.12167 |
| الاتاحة: | http://dx.doi.org/10.1111/1759-7714.12167 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1759-7714.12167 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1759-7714.12167 |
| Rights: | http://creativecommons.org/licenses/by-nc/4.0/ |
| رقم الانضمام: | edsbas.F109B64 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/1759-7714.12167 |
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